Tutorial: best practices and considerations for mass-spectrometry-based protein biomarker discovery and validation

ES Nakayasu, M Gritsenko, PD Piehowski, Y Gao… - Nature …, 2021 - nature.com
Mass-spectrometry-based proteomic analysis is a powerful approach for discovering new
disease biomarkers. However, certain critical steps of study design such as cohort selection …

Data‐independent acquisition‐based SWATH‐MS for quantitative proteomics: a tutorial

C Ludwig, L Gillet, G Rosenberger, S Amon… - Molecular systems …, 2018 - embopress.org
Many research questions in fields such as personalized medicine, drug screens or systems
biology depend on obtaining consistent and quantitatively accurate proteomics data from …

Inulin fibre promotes microbiota-derived bile acids and type 2 inflammation

M Arifuzzaman, TH Won, TT Li, H Yano, S Digumarthi… - Nature, 2022 - nature.com
Dietary fibres can exert beneficial anti-inflammatory effects through microbially fermented
short-chain fatty acid metabolites,, although the immunoregulatory roles of most fibre diets …

Missing value imputation approach for mass spectrometry-based metabolomics data

R Wei, J Wang, M Su, E Jia, S Chen, T Chen, Y Ni - Scientific reports, 2018 - nature.com
Missing values exist widely in mass-spectrometry (MS) based metabolomics data. Various
methods have been applied for handling missing values, but the selection can significantly …

Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry

BC Collins, CL Hunter, Y Liu, B Schilling… - Nature …, 2017 - nature.com
Quantitative proteomics employing mass spectrometry is an indispensable tool in life
science research. Targeted proteomics has emerged as a powerful approach for …

Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen-treated three-dimensional liver …

R Bruderer, OM Bernhardt, T Gandhi… - Molecular & Cellular …, 2015 - ASBMB
The data-independent acquisition (DIA) approach has recently been introduced as a novel
mass spectrometric method that promises to combine the high content aspect of shotgun …

[HTML][HTML] A multi-network approach identifies protein-specific co-expression in asymptomatic and symptomatic Alzheimer's disease

NT Seyfried, EB Dammer, V Swarup, D Nandakumar… - Cell systems, 2017 - cell.com
Here, we report proteomic analyses of 129 human cortical tissues to define changes
associated with the asymptomatic and symptomatic stages of Alzheimer's disease (AD) …

Accounting for the multiple natures of missing values in label-free quantitative proteomics data sets to compare imputation strategies

C Lazar, L Gatto, M Ferro, C Bruley… - Journal of proteome …, 2016 - ACS Publications
Missing values are a genuine issue in label-free quantitative proteomics. Recent works have
surveyed the different statistical methods to conduct imputation and have compared them on …

Diagnostics and correction of batch effects in large‐scale proteomic studies: a tutorial

J Čuklina, CH Lee, EG Williams, T Sajic… - Molecular systems …, 2021 - embopress.org
Advancements in mass spectrometry‐based proteomics have enabled experiments
encompassing hundreds of samples. While these large sample sets deliver much‐needed …

A systematic evaluation of normalization methods in quantitative label-free proteomics

T Välikangas, T Suomi, LL Elo - Briefings in bioinformatics, 2018 - academic.oup.com
To date, mass spectrometry (MS) data remain inherently biased as a result of reasons
ranging from sample handling to differences caused by the instrumentation. Normalization is …