The field of protein design has made remarkable progress over the past decade. Historically,
the low reliability of purely structure-based design methods limited their application, but …

Enzymes are efficient and specific catalysts for many essential reactions in biotechnological
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …

Although flavin-dependent halogenases (FDHs) are attractive for C–H bond activation, their
applications are limited due to low turnover and stability. We have previously shown that …

Given prominent physicochemical similarities between H2O2 and water, we report a new
strategy for promoting the peroxygenase activity of P450 enzymes by engineering their …

Activation heat capacity is emerging as a crucial factor in enzyme thermoadaptation, as
shown by the non-Arrhenius behaviour of many natural enzymes. However, its physical …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …

The experimental characterization and computational prediction of protein structures has
become increasingly rapid and precise. However, the analysis of protein structures often …

Carbon monoxide dehydrogenase (CODH), formate dehydrogenase (FDH), hydrogenase
(H2ase), and nitrogenase (N2ase) are crucial enzymatic catalysts that facilitate the …

Conspectus Cytochrome P450 monooxygenase is a versatile oxidizing enzyme with great
potential in synthetic chemistry and biology. However, the dependence of its catalytic …