Cancer is one of the primary causes of worldwide human deaths. Most cancer patients
receive chemotherapy and radiotherapy, but these treatments are usually only partially …

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated proteins (CRISPR/Cas) systems, regarded as “God's scissors”, have recently …

Gene expression is in part controlled by cis-regulatory elements (CREs) such as enhancers
and repressive elements. Anecdotal evidence has indicated that a CRE and a promoter …

Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful
technologies to synthetically reshape the human epigenome and gene expression. Despite …

CRISPR-based gene activation (CRISPRa) is a strategy for upregulating gene expression
by targeting promoters or enhancers in a tissue/cell-type specific manner. Here, we describe …

Liquid–liquid phase separation (LLPS) plays a critical role in regulating gene transcription
via the formation of transcriptional condensates. However, LLPS has not been reported to be …

Osteoarthritis (OA) is one of the most common degenerative joint diseases worldwide,
causing pain, disability, and decreased quality of life. The balance between regeneration …

Despite extensive studies on the chromatin landscape of exhausted T cells, the
transcriptional wiring underlying the heterogeneous functional and dysfunctional states of …

Technologies for gene activation are valuable tools for the study of gene functions and have
a wide range of potential applications in bioengineering and medicine. In contrast to existing …

Cas12a CRISPR technology, unlike Cas9, allows for facile multiplexing of guide RNAs from
a single transcript, simplifying combinatorial perturbations. While Cas12a has been …