Light-sheet fluorescence microscopy is a rapidly growing technique that has gained
tremendous popularity in the life sciences owing to its high-spatiotemporal resolution and …

In this Review, we survey recent developments in the emerging field of high-speed variable-
z-focus optical elements, which are driving important innovations in advanced imaging and …

After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …

Rapid, high-resolution volumetric imaging without moving heavy objectives or disturbing
delicate samples remains challenging. Pupil-matched remote focusing offers a promising …

The spectral dispersion of ultrashort pulses allows the simultaneous focusing of light in both
space and time, which creates so-called spatiotemporal foci. Such space–time coupling may …

Recent advances brought the performance of MEMS-based varifocal mirrors to levels
comparable to conventional ultra-high-speed focusing devices. Varifocal mirrors are …

We present IsoSense, a wavefront sensing method that mitigates sample dependency in
image-based sensorless adaptive optics applications in microscopy. Our method employs …

In optical microscopy, the slow axial scanning rate of the objective or the sample has
traditionally limited the speed of volumetric imaging. Recently, by conjugating either a …

Dynamic axial focusing functionality has recently experienced widespread incorporation in
microscopy, augmented/virtual reality (AR/VR), adaptive optics and material processing …

Significance: Light-field microscopy (LFM) enables high signal-to-noise ratio (SNR) and light
efficient volume imaging at fast frame rates. Voltage imaging with genetically encoded …