DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

The polymerase chain reaction (PCR) is increasingly used as the standard method for
detection and characterization of microorganisms and genetic markers in a variety of sample …

The use of environmental DNA (eDNA) to determine the presence and distribution of aquatic
organisms has become an important tool to monitor and investigate freshwater communities …

PCR is an important and powerful tool in several fields, including clinical diagnostics, food
analysis, and forensic analysis. In theory, PCR enables the detection of one single cell or …

Real-time polymerase chain reaction (qPCR) is the cornerstone of DNA analysis, enabling
detection and quantification of minute nucleic acid amounts. However, PCR-based analysis …

Inhibition remains the greatest methodological challenge in molecular analysis of buried
biological remains. Inhibitory compounds associated with soil environments comprise …

DNA typing of degraded DNA samples can be a challenging task when using the current
commercially available multiplex short tandem repeat (STR) analysis kits. However, the …

Reverse transcriptase (RT) enzymes are indispensable tools for interrogating diverse
aspects of RNA metabolism and transcriptome composition. Due to the growing interest in …

The intimate interaction between plant host and associated microorganisms is crucial in
determining plant fitness, and can foster improved tolerance to abiotic stresses and …

Inhibition is problematic in many applications of PCR, particularly those involving degraded
or low amounts of template DNA, when simply diluting the extract is undesirable. Two basic …