A comprehensive summary of a decade development of the recombinase polymerase amplification

J Li, J Macdonald, F Von Stetten - Analyst, 2019 - pubs.rsc.org
Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …

Onsite detection of plant viruses using isothermal amplification assays

AI Bhat, R Aman, M Mahfouz - Plant biotechnology journal, 2022 - Wiley Online Library
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

Light-Switch Electrochemiluminescence-Driven microfluidic sensor for rapid and sensitive detection of Mpox virus

Y Fu, X Chen, W Song, J Kuang, W Wu, X Yang… - Chemical Engineering …, 2024 - Elsevier
Rapid, real-time, and accurate detection of pathogenic nucleic acids is crucial for the control
and treatment of Mpox virus infection; however, classical detection methods are limited by …

Efficient, rapid, and sensitive detection of plant RNA viruses with one-pot RT-RPA–CRISPR/Cas12a assay

R Aman, A Mahas, T Marsic, N Hassan… - Frontiers in …, 2020 - frontiersin.org
Most viruses that infect plants use RNA to carry their genomic information; timely and robust
detection methods are crucial for efficient control of these diverse pathogens. The RNA …

Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a‐based visual assay

J Jiao, K Kong, J Han, S Song, T Bai… - Plant biotechnology …, 2021 - Wiley Online Library
Co‐infection of apple trees with several viruses/viroids is common and decreases fruit yield
and quality. Accurate and rapid detection of these viral pathogens helps to reduce losses …

Review of empirical and emerging breeding methods and tools for yam (Dioscorea spp.) improvement: Status and prospects

K Darkwa, B Olasanmi, R Asiedu, A Asfaw - Plant Breeding, 2020 - Wiley Online Library
Yam (Dioscorea spp.) is an important tuber crop with tremendous potential as a functional
food in the tropics and subtropics. However, the crop has not shown progressive productivity …

Establishment of a one-step reverse transcription recombinase polymerase amplification assay for the detection of potato virus S

R Kumar, P Kaundal, RK Tiwari, S Siddappa… - Journal of Virological …, 2022 - Elsevier
Potato virus S (PVS) is a noteworthy threat to the propagation of healthy seed potatoes.
Accurate and speedy detection is critical for effective PVS management. In the present study …

Molecular approaches for low-cost point-of-care pathogen detection in agriculture and forestry

P Baldi, N La Porta - Frontiers in Plant Science, 2020 - frontiersin.org
Early detection of plant diseases is a crucial factor to prevent or limit the spread of a rising
infection that could cause significant economic loss. Detection test on plant diseases in the …

Rapid and sensitive detection of potato virus X by one-step reverse transcription-recombinase polymerase amplification method in potato leaves and dormant tubers

R Kumar, P Kaundal, RK Tiwari, S Siddappa… - Molecular and cellular …, 2021 - Elsevier
Potato virus X (PVX), is a serious threat to global potato production. A simple and rapid
detection method is imperative for PVX diagnosis and early management. In this study, an …

Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of "Candidatus …

DK Ghosh, SB Kokane, AD Kokane, AJ Warghane… - PloS one, 2018 - journals.plos.org
Huanglongbing (HLB) or citrus greening is highly destructive disease that is affecting the
citrus industry worldwide and it has killed millions of citrus plants globally. HLB is caused by …