Mycoplasma pneumoniae Infections: Pathogenesis and Vaccine Development

Z Jiang, S Li, C Zhu, R Zhou, PHM Leung - Pathogens, 2021 - mdpi.com
Mycoplasma pneumoniae is a major causative agent of community-acquired pneumonia
which can lead to both acute upper and lower respiratory tract inflammation, and …

Optimising expression and extraction of recombinant proteins in plants

RJ Coates, MT Young, S Scofield - Frontiers in Plant Science, 2022 - frontiersin.org
Recombinant proteins are of paramount importance for research, industrial and medical use.
Numerous expression chassis are available for recombinant protein production, and while …

Inteins in science: evolution to application

A Nanda, SS Nasker, A Mehra, S Panda, S Nayak - Microorganisms, 2020 - mdpi.com
Inteins are mobile genetic elements that apply standard enzymatic strategies to excise
themselves post-translationally from the precursor protein via protein splicing. Since their …

Compendium on food crop plants as a platform for pharmaceutical protein production

A Gerszberg, K Hnatuszko-Konka - International Journal of Molecular …, 2022 - mdpi.com
Tremendous advances in crop biotechnology related to the availability of molecular tools
and methods developed for transformation and regeneration of specific plant species have …

SPI “sandwich”: combined SUMO‐peptide‐Intein expression system and isolation procedure for improved stability and yield of peptides

T Lamer, MJ van Belkum, A Wijewardane… - Protein …, 2022 - Wiley Online Library
Recombinant peptide production in Escherichia coli is often accomplished through cloning
and expression of a fusion protein. The fusion protein partner generally has two …

Chromatographic single-step purification of tagless proteins using gp41-1 split inteins

M Knapp, V Kohl, T Best, O Rammo… - … in Bioengineering and …, 2024 - frontiersin.org
The current trend in biopharmaceutical drug manufacturing is towards increasing potency
and complexity of products such as peptide scaffolds, oligonucleotides and many more …

Inteins: A Swiss army knife for synthetic biology

S Anastassov, M Filo, M Khammash - Biotechnology Advances, 2024 - Elsevier
Inteins are proteins found in nature that execute protein splicing. Among them, split inteins
stand out for their versatility and adaptability, presenting creative solutions for addressing …

Engineering cell‐free protein synthesis for high‐yield production and human serum activity assessment of asparaginase: toward on‐demand treatment of acute …

JP Hunt, KM Wilding, RJ Barnett… - Biotechnology …, 2020 - Wiley Online Library
Acute lymphocytic leukemia (ALL) is a common childhood cancer in the United States, with
over 6000 new cases diagnosed each year. Administration of bacterial asparaginase …

Single-step non-chromatographic purification of recombinant mammalian proteins using a split intein ELP tag system

SV Prabhala, DW Wood - Protein Chromatography: Methods and Protocols, 2023 - Springer
Glycoprotein therapeutics are currently used by large patient populations and generate
significant revenue for the biopharmaceutical industry. These therapeutic proteins are …

Disease burden and macrolide resistance of Mycoplasma pneumoniae infection in adults in Asia-Pacific region

CC Lai, CC Hsueh, CK Hsu, YW Tsai… - International Journal of …, 2024 - Elsevier
Abstract In the Asia-Pacific region, Mycoplasma pneumoniae (MP) could be a notable
pathogen responsible for adult community-acquired pneumonia (CAP), with varying …