[HTML][HTML] Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global …

KM Ruppert, RJ Kline, MS Rahman - Global Ecology and Conservation, 2019 - Elsevier
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity
wherein samples are taken from the environment via water, sediment or air from which DNA …

Diet tracing in ecology: Method comparison and selection

JM Nielsen, EL Clare, B Hayden… - Methods in Ecology …, 2018 - Wiley Online Library
Determining diet is a key prerequisite for understanding species interactions, food web
structure and ecological dynamics. In recent years, there has been considerable …

Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive

D Hering, A Borja, JI Jones, D Pont, P Boets… - Water research, 2018 - Elsevier
Assessment of ecological status for the European Water Framework Directive (WFD) is
based on “Biological Quality Elements”(BQEs), namely phytoplankton, benthic flora, benthic …

An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data

LE Drake, JP Cuff, RE Young… - Methods in Ecology …, 2022 - Wiley Online Library
Metabarcoding provides a powerful tool for investigating biodiversity and trophic
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …

Tag jumps illuminated–reducing sequence‐to‐sample misidentifications in metabarcoding studies

IB Schnell, K Bohmann… - Molecular ecology …, 2015 - Wiley Online Library
Metabarcoding of environmental samples on second‐generation sequencing platforms has
rapidly become a valuable tool for ecological studies. A fundamental assumption of this …

Promises and pitfalls of using high‐throughput sequencing for diet analysis

A Alberdi, O Aizpurua, K Bohmann… - Molecular ecology …, 2019 - Wiley Online Library
The application of high‐throughput sequencing‐based approaches to DNA extracted from
environmental samples such as gut contents and faeces has become a popular tool for …

Universal and blocking primer mismatches limit the use of high‐throughput DNA sequencing for the quantitative metabarcoding of arthropods

J Piñol, G Mir, P Gomez‐Polo… - Molecular ecology …, 2015 - Wiley Online Library
The quantification of the biological diversity in environmental samples using high‐
throughput DNA sequencing is hindered by the PCR bias caused by variable primer …

Quantitative DNA metabarcoding: improved estimates of species proportional biomass using correction factors derived from control material

AC Thomas, BE Deagle, JP Eveson… - Molecular ecology …, 2016 - Wiley Online Library
DNA metabarcoding is a powerful new tool allowing characterization of species
assemblages using high‐throughput amplicon sequencing. The utility of DNA …

Overcoming the pitfalls of merging dietary metabarcoding into ecological networks

JP Cuff, FM Windsor, MPTG Tercel… - Methods in Ecology …, 2022 - Wiley Online Library
The construction of increasingly detailed species interaction networks is extending the
potential applications of network ecology, providing an opportunity to understand complex …

DNA metabarcoding of insects and allies: an evaluation of primers and pipelines

GJ Brandon-Mong, HM Gan, KW Sing… - Bulletin of …, 2015 - cambridge.org
Metabarcoding, the coupling of DNA-based species identification and high-throughput
sequencing, offers enormous promise for arthropod biodiversity studies but factors such as …