The present review deals with prefractionation protocols used in proteomic investigation in
preparation for mass spectrometry (MS) or two‐dimensional electrophoresis (2‐DE) map …

The plant mitochondrial proteome might contain as many as 2000–3000 different gene
products, each of which might undergo post-translational modification. Recent studies using …

This laboratory guide for successful electrophoretic separations is divided into two parts to
provide readers with a thorough presentation of the fundamentals followed by a detailed …

In this study yeast mitochondria were used as a model system to apply, evaluate, and
integrate different genomic approaches to define the proteins of an organelle. Liquid …

Mitochondria perform several fundamental cellular processes in higher eukaryotes including
oxidative phosphorylation, Fe/S cluster formation and apoptosis. Dysfunction of the …

To attain a comprehensive membrane proteome of two strains of Corynebacterium
glutamicum (l-lysine-producing and the characterized model strains), both sample …

Proteomics, the characterization of the proteome, is conceptually simple but technically
challenging. Development of such technologies as mass spectrometry, multidimensional …

In eucaryotes, glycolytic enzymes are classically regarded as being localised in the cytosol.
Recently, we have shown that part of the cellular pool of the glycolytic enzyme, enolase, is …

Still the only concise practical guide to laboratory experiments in proteomics, this new
edition now also covers DIGE technology and liquid-chromatography, while the …

The identification, quantitation and global characterisation of all proteins within a given
proteome are extremely challenging. This is due to the absolute detection limits of …