Cells navigate environments, communicate and build complex patterns by initiating gene
expression in response to specific signals. Engineers seek to harness this capability to …

The past several years have witnessed an increased presence of control theoretic concepts
in synthetic biology. This review presents an organized summary of how these control …

Engineered genetic circuits for mammalian cells often require extensive fine-tuning to
perform as intended. We present a robust, general, scalable system, called'Boolean logic …

Much of contemporary synthetic biology research relies on the use of bacterial chassis for
plugging-in and plugging-out genetic circuits and new-to-nature functionalities. However …

A common approach to design genetic circuits is to compose gene expression cassettes
together. While appealing, this modular approach is challenged by the fact that expression …

Large synthetic genetic circuits require the simultaneous expression of many regulators.
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …

Natural genetic circuits enable cells to make sophisticated digital decisions. Building equally
complex synthetic circuits in eukaryotes remains difficult, however, because commonly used …

Synthetic biology has the potential to bring forth advanced genetic devices for applications
in healthcare and biotechnology. However, accurately predicting the behavior of engineered …

Synthetic genetic systems share resources with the host, including machinery for
transcription and translation. Phage RNA polymerases (RNAP s) decouple transcription from …

The behavior of gene modules in complex synthetic circuits is often unpredictable 1, 2, 3, 4.
After joining modules to create a circuit, downstream elements (such as binding sites for a …