qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection
and quantification of RNA targets and is being utilized increasingly in novel clinical …

In this era of the Human Genome Project, quantitation of gene expression in tumor or host
cells is of paramount importance for investigating the gene patterns responsible for cancer …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

Background: The antiviral effect of anti-influenza drugs such as zanamivir may be
demonstrated in patients as an increased rate of decline in viral load over a time course of …

Background: The detection of circulating tumor cells (CTC) in patients with melanoma
represents an appealing prognostic tool, but no consensus exists on this topic. We aimed to …

Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as
the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive …

The worldwide incidence of malignant melanoma has been increasing during the past
decade and is a public health concern because this disease accounts for up to 90% of …

Yellow fever virus quantitation is performed routinely by cultivation of virus containing
samples using susceptible cells. Counting of the resulting plaques provides a marker for the …

We present a review of current literature concerning the significance of serological markers
in melanoma patients. Results for cytokines, cytokine receptors, cell adhesion molecules …

Real‐time PCR is becoming the method of choice for precise quantification of minute
amounts of nucleic acids. For proper comparison of samples, almost all quantification …