Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …

[HTML][HTML] ITS alchemy: on the use of ITS as a DNA marker in fungal ecology

H Kauserud - Fungal Ecology, 2023 - Elsevier
High throughput sequencing of PCR amplicons derived from environmental DNA (aka DNA
metabarcoding) has become an integral part of fungal ecology, enabling in-depth …

Characterization and demonstration of mock communities as control reagents for accurate human microbiome community measurements

DM Tourlousse, K Narita, T Miura, A Ohashi… - Microbiology …, 2022 - Am Soc Microbiol
Standardization and quality assurance of microbiome community analysis by high-
throughput DNA sequencing require widely accessible and well-characterized reference …

The skin microbiome: current techniques, challenges, and future directions

TM Santiago-Rodriguez, B Le François, JM Macklaim… - Microorganisms, 2023 - mdpi.com
Skin acts as a barrier that promotes the colonization of bacteria, fungi, archaea, and viruses
whose membership and function may differ depending on the various specialized niches or …

A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution

F Ijaz, R Nakazato, M Setou, K Ikegami - Scientific Reports, 2022 - nature.com
The introduction of small insertion/deletion (indel) mutations in the coding region of genes
by the site-specific nucleases such as Cas9 allows researchers to obtain frameshift null …

Methods in Nutrition & Gut Microbiome Research: An American Society for Nutrition Satellite Session [13 October 2022]

The microbial cells colonizing the human body form an ecosystem that is integral to the
regulation and maintenance of human health. Elucidation of specific associations between …

[PDF][PDF] Methods in Nutrition & Gut Microbiome Research: An American Society for Nutrition Satellite Session [13 October 2022]. Nutrients 2023, 15, 2451

RL Hughes, CL Frankenfeld, DM Gohl, C Huttenhower… - 2023 - lirias.kuleuven.be
The microbial cells colonizing the human body form an ecosystem that is integral to the
regulation and maintenance of human health. Elucidation of specific associations between …

Cloning and Characterization of Cyp7a1 and Cyp27a1 Genes from the Non-Parasitic Japanese Lamprey Lethenteron reissneri

M Morii, T Hebiguchi, R Watanabe, H Yoshino… - Zoological science, 2023 - BioOne
Two cytochrome P450 genes homologous to human CYP7A1 and CYP27A1 were cloned
from the non-parasitic Japanese lamprey Lethenteron reissneri. Lamprey cyp7a1 mRNA …

Estimateurs de biodiversité robustes pour le métabarcoding

S Moinard - 2023 - theses.hal.science
Du fait de l'activité humaine, la biodiversité connaît un bouleversement rapide à l'échelle
mondiale: extinction d'espèces, diminution des populations, introduction d'espèces …

GEM-Gate: A Low-Cost, Flexible Approach to BioBrick Assembly

C Bower, C Harbin, D Camenares - DNA, 2023 - mdpi.com
Rapid and modular assembly of DNA parts is crucial to many synthetic biologists. This can
be achieved through Golden Gate assembly, which often requires purchase and delivery of …