For centuries, cell biology has been based on light microscopy and at the same time been
limited by its optical resolution. However, several new technologies have been developed …

Abstract In 1873, Ernst Abbe discovered that features closer than∼ 200 nm cannot be
resolved by lens-based light microscopy. In recent years, however, several new far-field …

Optical approaches for observing the dynamics of single molecules have required pico-to
nanomolar concentrations of fluorophore in order to isolate individual molecules. However …

Since the earliest examination of cellular structures, biologists have been fascinated by
observing cells using light microscopy. The advent of fluorescent labeling technologies plus …

We demonstrate live-cell super-resolution imaging using photoactivated localization
microscopy (PALM). The use of photon-tolerant cell lines in combination with the high …

Recruitment of receptor proteins to lipid rafts has been proposed as an important
mechanism to regulate their cellular function. In particular, rafts have been implicated in …

Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been
substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical …

The direct observation of nanoscale objects is a challenging task for optical microscopy
because the scattering from an individual nanoparticle is typically weak at optical …

In the last decade emerging single‐molecule fluorescence‐spectroscopy tools have been
developed and adapted to analyze individual molecules under various conditions. Single …

The manipulation of photons in structures smaller than the wavelength of light is central to
the development of nanoscale integrated photonic systems for computing, communications …