Tools and techniques for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/COVID-19 detection

SH Safiabadi Tali, JJ LeBlanc, Z Sadiq… - Clinical microbiology …, 2021 - Am Soc Microbiol
SUMMARY The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory disease coronavirus 2 (SARS-CoV-2), has led to millions of confirmed cases and …

The emerging concern and interest SARS-CoV-2 variants

E Janik, M Niemcewicz, M Podogrocki, I Majsterek… - Pathogens, 2021 - mdpi.com
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) responsible for
coronavirus disease 2019 (COVID-19) was discovered in December 2019 in Wuhan, China …

Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

T Dong, M Wang, J Liu, P Ma, S Pang, W Liu… - Chemical Science, 2023 - pubs.rsc.org
The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
has induced severe public healthcare issues and weakened the global economy …

[HTML][HTML] Real-time SARS-CoV-2 genotyping by high-throughput multiplex PCR reveals the epidemiology of the variants of concern in Qatar

MR Hasan, MKR Kalikiri, F Mirza, S Sundararaju… - International Journal of …, 2021 - Elsevier
Complementing whole genome sequencing strategies with high-throughput multiplex RT-
qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 …

[HTML][HTML] SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B. 1.1. 7 lineage in a commercial multiplex PCR assay

P Wollschläger, D Todt, N Gerlitz, S Pfaender… - Clinical Microbiology …, 2021 - Elsevier
Objectives Detection and surveillance of SARS-CoV-2 is of eminent importance, particularly
due to the rapid emergence of variants of concern (VOCs). In this study we evaluated if a …

Nucleocapsid (N) gene mutations of SARS-CoV-2 can affect real-time RT-PCR diagnostic and impact false-negative results

JCC Lesbon, MD Poleti, EC de Mattos Oliveira… - Viruses, 2021 - mdpi.com
The current COVID-19 pandemic demands massive testing by Real-time RT-PCR (Reverse
Transcription Polymerase Chain Reaction), which is considered the gold standard …

Influenza A, influenza B, and SARS-CoV-2 similarities and differences–a focus on diagnosis

A Havasi, S Visan, C Cainap, SS Cainap… - Frontiers in …, 2022 - frontiersin.org
In late December 2019, the first cases of viral pneumonia caused by an unidentified
pathogen were reported in China. Two years later, SARS-CoV-2 was responsible for almost …

False-negative RT-PCR findings and double mutant variant as factors of an overwhelming second wave of COVID-19 in India: an emerging global health disaster

H Jindal, S Jain, TK Suvvari, LVS Kutikuppala… - SN comprehensive …, 2021 - Springer
RT-PCR is considered to be the standard gold diagnostic test for detecting COVID-19
causing SARS-CoV-2. Recent reports and recent pieces of evidence from scientific …

Single-point mutations in the N gene of SARS-CoV-2 adversely impact detection by a commercial dual target diagnostic assay

S Miller, T Lee, A Merritt, T Pryce, A Levy… - Microbiology …, 2021 - Am Soc Microbiol
Accurate and rapid diagnostic tests are a critical component for the early diagnosis of severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and of the overall control strategy …

Sars-Cov-2 detection methods

A Lino, MA Cardoso, HMR Gonçalves, P Martins-Lopes - Chemosensors, 2022 - mdpi.com
A fast and highly specific detection of COVID-19 infections is essential in managing the virus
dissemination networks. The most relevant technologies developed for SARS-CoV-2 …