Microbial-derived natural products (NPs) and their derivative products are of great
importance and used widely in many fields, especially in pharmaceutical industries …

Serving a robust platform for reverse genetics enabling the in vivo study of gene functions
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …

Synthetic biology offers the opportunity to build solutions for improved capture and
conversion of carbon dioxide (CO2) that outcompete those evolved by nature. Here we …

Abstract One-carbon (C1) substrates, such as methanol or formate, are attractive feedstocks
for circular bioeconomy. These substrates are typically converted into formaldehyde, serving …

Gene-drive systems in diploid organisms bias the inheritance of one allele over another.
CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site …

BACKGROUND The Mediterranean fruit fly (medfly), Ceratitis capitata Wiedemann, is a
major pest affecting fruit and vegetable production worldwide, whose control is mainly based …

Microbial β-glucuronidases (GUSs) cause severe gut toxicities that limit the efficacy of
cancer drugs and other therapeutics. Selective inhibitors of bacterial GUS have been shown …

Background Efficient microbial production of chemicals is often hindered by the cytotoxicity
of the products or by the pathogenicity of the host strains. Hence 2, 3-butanediol, an …

Transcriptional pausing aids gene regulation by cellular RNA polymerases (RNAPs). A
surface-exposed domain inserted into the catalytic trigger loop (TL) of Escherichia coli …

N 1-methylation of G37 is required for a subset of tRNAs to maintain the translational
reading-frame. While loss of m 1 G37 increases ribosomal+ 1 frameshifting, whether it incurs …