Multiplex staining by sequential immunostaining and antibody removal on routine tissue sections

MM Bolognesi, M Manzoni, CR Scalia… - … of Histochemistry & …, 2017 - journals.sagepub.com
Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in
situ, has raised considerable interest. The methods proposed include the use of labeled …

B cells secrete functional antigen-specific IgG antibodies on extracellular vesicles

C Rival, M Mandal, K Cramton, H Qiao, M Arish… - Scientific reports, 2024 - nature.com
B cells and the antibodies they produce are critical in host defense against pathogens and
contribute to various immune-mediated diseases. B cells responding to activating signals in …

Towards a pan marsupial sero-immunological tool in the demanding field of wildlife serology: Marsupial immunoglobulin-binding capability with protein A/G, protein L …

KLDTD Liyanage, PK Vaz, A Jabbar, J Hufschmid - Plos one, 2023 - journals.plos.org
Detection of infections in wildlife species is increasingly important to reduce the risk of
spreading zoonotic and economically important parasites, understand disease …

Site-specific conjugation of fibroblast growth factor 2 (FGF2) based on incorporation of alkyne-reactive unnatural amino acid

KW Swiderska, A Szlachcic, A Czyrek… - Bioorganic & medicinal …, 2017 - Elsevier
Recent advances in site-specific protein modification include the increasingly popular
incorporation of unnatural amino acid (s) using amber codon, a method developed by …

Increasing the efficiency of gene editing with CRISPR-Cas9 via concurrent expression of the Beta protein

W Zhao, Y Guo - International Journal of Biological Macromolecules, 2024 - Elsevier
Escherichia coli has emerged as an important host for the production of biopharmaceuticals
or other industrially relevant molecules. An efficient gene editing tool is indispensable for …

Covalent and oriented immobilization of antibodies through systematic modification of photoactivatable RNA hybrid aptamers

CH Jeon, TH Ha - Bioconjugate Chemistry, 2022 - ACS Publications
We report a covalent and well-oriented strategy of immobilization of antibodies using
photoactivatable Fc-binding RNA aptamers (FcBAs). We prepared several types of FcBAs …

First site-specific conjugation method for native goat IgG antibodies via glycan remodeling at the conserved Fc region

ME Dolan, A Sadiki, L Wang, Y Wang… - Antibody …, 2024 - academic.oup.com
Despite their triumph in treating human diseases, antibody therapies for animals have
gained momentum more slowly. However, the first approvals of animal antibodies for …

Acid pH promotes bispecific antibody formation by the redox procedure

J Gupta, M Hoque, MF Ahmad, RH Khan… - International journal of …, 2019 - Elsevier
Bispecific antibodies (BsAbs), are potential theranostics. Chemical procedures of
preparation of BsAbs, in which two monospecific antibodies are split into half molecules and …

Development of Quantitative Intensity-Based Single-Molecule Assays

B Croop - 2021 - stars.library.ucf.edu
Fluorescence microscopy has emerged as a popular and powerful tool within biology
research, owing to its exceptional signal contrast, specificity, and the versatility of the various …

Μελέτη της χρόνιας νόσου των νεφρών μέσω πρωτεομικής τοπολογικής και single-cell ανάλυσης ιστολογικών δειγμάτων

Μ Χρυσοπούλου - 2020 - dspace.aua.gr
Η χρόνια νόσος των νεφρών αποτελεί μία πολυπαραγοντική συστημική ασθένεια που
περιλαμβάνει ένα εύρος παθολογικών καταστάσεων των νεφρών, με πολλαπλές αρχικές …