The advent of heme during evolution allowed organisms possessing this compound to
safely and efficiently carry out a variety of chemical reactions that otherwise were difficult or …

Transposon insertion sequencing (TIS) is a powerful approach that can be extensively
applied to the genome-wide definition of loci that are required for bacterial growth under …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

The combination of high-density transposon-mediated mutagenesis and high-throughput
sequencing has led to significant advancements in research on essential genes, resulting in …

Phosphoribosyl diphosphate (PRPP) is an important intermediate in cellular metabolism.
PRPP is synthesized by PRPP synthase, as follows: ribose 5-phosphate+ ATP→ PRPP+ …

Resistance to antimicrobial drugs is currently a serious threat to human health.
Consequently, we are facing an urgent need for new antimicrobial drugs acting with original …

Cell-free protein expression plays an important role in biochemical research. However, only
recent developments led to new methods to rapidly synthesize preparative amounts of …

High-resolution three-dimensional structures of essential Mycobacterium tuberculosis (Mtb)
proteins provide templates for TB drug design, but are available for only a small fraction of …

The universally conserved enzyme phosphoribosyl pyrophosphate synthetase (PRPS)
assembles filaments in evolutionarily diverse organisms. PRPS is a key regulator of …

Abstract Development of new anti-bacterial agents acting upon underexploited targets and
thus evading known mechanisms of resistance is the need of the hour. The highly conserved …