Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
[HTML][HTML] Current status and challenges of DNA base editing tools
CRISPR-mediated DNA base editors, which include cytosine base editors (CBEs) and
adenine base editors (ABEs), are promising tools that can induce point mutations at desired …
adenine base editors (ABEs), are promising tools that can induce point mutations at desired …
[HTML][HTML] Genome-wide CRISPR off-target prediction and optimization using RNA-DNA interaction fingerprints
Q Chen, G Chuai, H Zhang, J Tang, L Duan… - Nature …, 2023 - nature.com
The powerful CRISPR genome editing system is hindered by its off-target effects, and
existing computational tools achieved limited performance in genome-wide off-target …
existing computational tools achieved limited performance in genome-wide off-target …
[HTML][HTML] PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
Background The rapid expansion of the CRISPR toolbox through tagging effector domains
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors
A Zhang, T Shan, Y Sun, Z Chen, J Hu… - Plant Biotechnology …, 2023 - Wiley Online Library
CRISPRbased directed evolution is an effective breeding biotechnology to improve
agronomic traits in plants. However, its gene diversification is still limited using individual …
agronomic traits in plants. However, its gene diversification is still limited using individual …
Predicting base editing outcomes using position-specific sequence determinants
CRISPR/Cas base editors promise nucleotide-level control over DNA sequences, but the
determinants of their activity remain incompletely understood. We measured base editing …
determinants of their activity remain incompletely understood. We measured base editing …
[HTML][HTML] Single-base resolution: increasing the specificity of the CRISPR-Cas system in gene editing
R Rabinowitz, D Offen - Molecular Therapy, 2021 - cell.com
The CRISPR-Cas system holds great promise in the treatment of diseases caused by
genetic variations. The Cas protein, an RNA-guided programmable nuclease, generates a …
genetic variations. The Cas protein, an RNA-guided programmable nuclease, generates a …
ModelTeller: model selection for optimal phylogenetic reconstruction using machine learning
Statistical criteria have long been the standard for selecting the best model for phylogenetic
reconstruction and downstream statistical inference. Although model selection is regarded …
reconstruction and downstream statistical inference. Although model selection is regarded …
[HTML][HTML] Genome editing and bioinformatics
Bioinformatics has become an indispensable technology in molecular biology for genome
editing. In this review, we outline various bioinformatic techniques necessary for genome …
editing. In this review, we outline various bioinformatic techniques necessary for genome …
[HTML][HTML] CrisPam: SNP-derived PAM analysis tool for allele-specific targeting of genetic variants using CRISPR-Cas systems
Clustered regularly interspaced short palindromic repeats (CRISPR) is a promising novel
technology that holds the potential of treating genetic diseases. Safety and specificity of the …
technology that holds the potential of treating genetic diseases. Safety and specificity of the …