In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

NOTE: In the version of this Review initially published, an author (B. Martin Hallberg) was left
off of the author list. This information has been added to the HTML and PDF versions of the …

After the global spread of the SARS-CoV-2 Omicron BA. 2, some BA. 2 subvariants,
including BA. 2.9. 1, BA. 2.11, BA. 2.12. 1, BA. 4, and BA. 5, emerged in multiple countries …

In vivo homologous recombination holds the potential for optimal molecular cloning,
however, current strategies require specialised bacterial strains or laborious protocols. Here …

Most proteins for structural biology studies are produced by high-level expression in
Escherichia coli. However, prokaryotic based expression systems fail to generate correctly …

Recombinant proteins are widely used today in many industries, including the
biopharmaceutical industry, and can be expressed in bacteria, yeasts, mammalian and …

Plant-based overexpression of heterologous proteins has attracted much interest and
development in recent years. To date, the most efficient vectors have been based on RNA …

Glycoproteins present special problems for structural genomic analysis because they often
require glycosylation in order to fold correctly, whereas their chemical and conformational …

Measles still remains a major cause of childhood morbidity and mortality worldwide.
Measles virus (MV) vaccines are highly successful, but the mechanism underlying their …

Producing soluble proteins in Escherichia coli is still a major bottleneck for structural
proteomics. Therefore, screening for soluble expression on a small scale is an attractive way …