Microfluidics for biotechnology: bridging gaps to foster microfluidic applications

V Ortseifen, M Viefhues, L Wobbe… - … in Bioengineering and …, 2020 - frontiersin.org
Microfluidics and novel lab-on-a-chip applications have the potential to boost
biotechnological research in ways that are not possible using traditional methods. Although …

The roles of nucleoid-associated proteins and topoisomerases in chromosome structure, strand segregation, and the generation of phenotypic heterogeneity in …

V Norris, C Kayser, G Muskhelishvili… - FEMS Microbiology …, 2023 - academic.oup.com
How to adapt to a changing environment is a fundamental, recurrent problem confronting
cells. One solution is for cells to organize their constituents into a limited number of spatially …

Deep learning automates the quantitative analysis of individual cells in live-cell imaging experiments

DA Van Valen, T Kudo, KM Lane… - PLoS computational …, 2016 - journals.plos.org
Live-cell imaging has opened an exciting window into the role cellular heterogeneity plays
in dynamic, living systems. A major critical challenge for this class of experiments is the …

Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

KM Wetmore, MN Price, RJ Waters, JS Lamson, J He… - MBio, 2015 - Am Soc Microbiol
Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach
to annotate gene function in bacteria, but existing protocols for TnSeq require laborious …

A global resource allocation strategy governs growth transition kinetics of Escherichia coli

DW Erickson, SJ Schink, V Patsalo, JR Williamson… - Nature, 2017 - nature.com
A grand challenge of systems biology is to predict the kinetic responses of living systems to
perturbations starting from the underlying molecular interactions. Changes in the nutrient …

Bet-hedging during bacterial diauxic shift

A Solopova, J Van Gestel… - Proceedings of the …, 2014 - National Acad Sciences
When bacteria grow in a medium with two sugars, they first use the preferred sugar and only
then start metabolizing the second one. After the first exponential growth phase, a short lag …

Memory and fitness optimization of bacteria under fluctuating environments

G Lambert, E Kussell - PLoS genetics, 2014 - journals.plos.org
Bacteria prudently regulate their metabolic phenotypes by sensing the availability of specific
nutrients, expressing the required genes for their metabolism, and repressing them after …

Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software

M Kaiser, F Jug, T Julou, S Deshpande, T Pfohl… - Nature …, 2018 - nature.com
Much is still not understood about how gene regulatory interactions control cell fate
decisions in single cells, in part due to the difficulty of directly observing gene regulatory …

The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate

D Chu, DJ Barnes - Scientific reports, 2016 - nature.com
Bi-phasic or diauxic growth is often observed when microbes are grown in a chemically
defined medium containing two sugars (for example glucose and lactose). Typically, the two …

The volumes and transcript counts of single cells reveal concentration homeostasis and capture biological noise

H Kempe, A Schwabe, F Crémazy… - Molecular biology of …, 2015 - Am Soc Cell Biol
Transcriptional stochasticity can be measured by counting the number of mRNA molecules
per cell. Cell-to-cell variability is best captured in terms of concentration rather than molecule …