Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Arrhythmias are common and contribute substantially to cardiovascular morbidity and
mortality. The underlying pathophysiology of arrhythmias is complex and remains …

Transcription activator-like effector nucleases (TALENs) are programmable nucleases that
join FokI endonuclease with the modular DNA-binding domain of TALEs. Although zinc …

Targeted modification of the pig genome can be challenging. Recent applications of the
CRISPR/Cas9 system hold promise for improving the efficacy of genome editing. When a …

Cardiovascular disease remains the leading cause of morbidity and mortality in the
developed world. In recent decades, extraordinary effort has been devoted to defining the …

The domestic pig has been widely used as an important large animal model. Precise and
efficient genetic modification in pig provides a great promise in biomedical research …

Animal models of human diseases play a critical role in medical research. Pigs are
anatomically and physiologically more like humans than are small rodents such as mice …

Zinc-finger nucleases (ZFNs) are powerful tools for producing gene knockouts (KOs) with
high efficiency. Whereas ZFN-mediated gene disruption has been demonstrated in …

One of the most powerful strategies to investigate biology we have as scientists, is the ability
to transfer genetic material in a controlled and deliberate manner between organisms. When …

Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth.
Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) …