Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics

S Ardui, A Ameur, JR Vermeesch… - Nucleic acids …, 2018 - academic.oup.com
Short read massive parallel sequencing has emerged as a standard diagnostic tool in the
medical setting. However, short read technologies have inherent limitations such as GC …

Review of clinical next-generation sequencing

S Yohe, B Thyagarajan - Archives of pathology & …, 2017 - meridian.allenpress.com
Context.—Next-generation sequencing (NGS) is a technology being used by many
laboratories to test for inherited disorders and tumor mutations. This technology is new for …

[HTML][HTML] One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

S Koren, AM Phillippy - Current opinion in microbiology, 2015 - Elsevier
Highlights•We describe three long-read sequencing technologies and their
characteristics.•We describe available algorithms for long-read assembly and their …

The present and future of de novo whole-genome assembly

J Sohn, JW Nam - Briefings in bioinformatics, 2018 - academic.oup.com
As the advent of next-generation sequencing (NGS) technology, various de novo assembly
algorithms based on the de Bruijn graph have been developed to construct chromosome …

Whole genome sequencing in clinical and public health microbiology

JC Kwong, N McCallum, V Sintchenko, BP Howden - Pathology, 2015 - Elsevier
Genomics and whole genome sequencing (WGS) have the capacity to greatly enhance
knowledge and understanding of infectious diseases and clinical microbiology. The growth …

Next-generation sequencing (NGS) in the microbiological world: How to make the most of your money

AT Vincent, N Derome, B Boyle, AI Culley… - Journal of …, 2017 - Elsevier
The Sanger sequencing method produces relatively long DNA sequences of unmatched
quality and has been considered for long time as the gold standard for sequencing DNA …

FMLRC: Hybrid long read error correction using an FM-index

JR Wang, J Holt, L McMillan, CD Jones - BMC bioinformatics, 2018 - Springer
Background Long read sequencing is changing the landscape of genomic research,
especially de novo assembly. Despite the high error rate inherent to long read technologies …

Vegetation biomass and soil moisture coregulate bacterial community succession under altered precipitation regimes in a desert steppe in northwestern China

X Na, H Yu, P Wang, W Zhu, Y Niu, J Huang - Soil Biology and …, 2019 - Elsevier
Given that soil water availability is the major limitation on plant growth in desert steppes,
uncovering the connections between belowground and aboveground communities under …

Complete chloroplast genome of Lilium ledebourii (Baker) Boiss and its comparative analysis: lights into selective pressure and adaptive evolution

M Sheikh-Assadi, R Naderi, M Kafi, R Fatahi… - Scientific Reports, 2022 - nature.com
Abstract Lilium ledebourii (Baker) Boiss is a rare species, which exhibits valuable traits.
However, before its genetic diversity and evolutionary were uncovered, its wild resources …

Primer ID validates template sampling depth and greatly reduces the error rate of next-generation sequencing of HIV-1 genomic RNA populations

S Zhou, C Jones, P Mieczkowski… - Journal of virology, 2015 - Am Soc Microbiol
Validating the sampling depth and reducing sequencing errors are critical for studies of viral
populations using next-generation sequencing (NGS). We previously described the use of …