Fusion proteins, understood as those created by joining two or more genes that originally
encoded independent proteins, have numerous applications in biotechnology, from …

Multi-enzyme complexes have the potential to achieve high catalytic efficiency for sequence
reactions due to their advantages in eliminating product inhibition, facilitating intermediate …

Flexibility or rigidity of the linker between two fused proteins is an important parameter that
affects the function of fusion proteins. In this study, we constructed a linker library with five …

Heparinases are enzymes that selectively cleave heparin and heparan sulfate chains, via
cleavage of the glycosidic linkage between hexosamines and uronic acids, producing …

Fusion strategy has been widely used to construct artificial multifunction proteins. The
flexibility or rigidity of linkers between two fused partners is an important parameter that …

Heparinase has attracted much attention because of its applications in pharmaceutical
industry. Herein, the heparinases III from Flavobacterium heparinum (FhepIII) and …

Extensive use of polyethylene terephthalate (PET) has brought about global environmental
problems. A recently reported PET hydrolase (PETase) discovered from Ideonella …

Naringinase (NGase) is a multi-compound enzyme with the subunits of α-L-rhamnosidase
(Rha) and β-D-glucosidase (BGL). The biotransformation of naringin into the more valuable …

Heparinases, including heparinases I–III (HepI, HepII, and HepIII, respectively), are
important tools for producing low‐molecular‐weight heparin, an improved anticoagulant …

Background Biosynthesis of l-tert-leucine (l-tle), a significant pharmaceutical intermediate,
by a cofactor regeneration system friendly and efficiently is a worthful goal all the time. The …