Methods for profiling genes at the single-cell level have revolutionized our ability to study
several biological processes and systems including development, differentiation, response …

Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

High-throughput amplicon sequencing of large genomic regions remains challenging for
short-read technologies. Here, we report a high-throughput amplicon sequencing approach …

Humans display substantial interindividual clinical variability after SARS-CoV-2 infection,–,
the genetic and immunological basis of which has begun to be deciphered. However, the …

Traditionally, the viral replication cycle is envisioned as a single, well-defined loop with four
major steps: attachment and entry into a target cell, replication of the viral genome …

The twenty-first century has seen the emergence of many epidemic and pandemic viruses,
with the most recent being the SARS-CoV-2-driven COVID-19 pandemic. As obligate …

Transcription and replication of the influenza virus RNA genome is catalyzed by the viral
heterotrimeric RNA-dependent RNA polymerase in the context of viral ribonucleoprotein …

Viral infection is usually studied at the population level by averaging over millions of cells.
However, infection at the single-cell level is highly heterogeneous, with most infected cells …

Ebola virus (EBOV) causes epidemics with high mortality yet remains understudied due to
the challenge of experimentation in high-containment and outbreak settings. Here, we used …

The human airway epithelium is the initial site of SARS-CoV-2 infection. We used flow
cytometry and single cell RNA-sequencing to understand how the heterogeneity of this …