Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements
J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …
and the availability of improved genetic tools/methods are making it more valuable than …
Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights
S Restrepo-Pineda, N O. Pérez… - FEMS Microbiology …, 2021 - academic.oup.com
Recombinant protein (RP) production from Escherichia coli has been extensively studied to
find strategies for increasing product yields. The thermoinducible expression system is …
find strategies for increasing product yields. The thermoinducible expression system is …
[HTML][HTML] The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli
S Restrepo-Pineda, N Sánchez-Puig, NO Pérez… - Applied Microbiology …, 2022 - Springer
The overproduction of recombinant proteins in Escherichia coli leads to insoluble
aggregates of proteins called inclusion bodies (IBs). IBs are considered dynamic entities that …
aggregates of proteins called inclusion bodies (IBs). IBs are considered dynamic entities that …
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coli
MA Meigouni, AA Mehrizi, A Fazaeli, S Zakeri… - Protein Expression and …, 2022 - Elsevier
Abstract Generative cell specific 1 (GCS1) or Hapless2 (Hap2) is a main transmission-
blocking vaccine (TBV) candidate against malaria. Experience has shown that this protein is …
blocking vaccine (TBV) candidate against malaria. Experience has shown that this protein is …
[HTML][HTML] Additivities for Soluble Recombinant Protein Expression in Cytoplasm of Escherichia coli
DL Atroshenko, EP Sergeev, DI Golovina, AA Pometun - Fermentation, 2024 - mdpi.com
Recombinant protein expression in Escherichia coli is a fundamental technique in molecular
biology and biotechnology. This review provides a comprehensive overview of various …
biology and biotechnology. This review provides a comprehensive overview of various …
[HTML][HTML] Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli
S Restrepo-Pineda, D Rosiles-Becerril… - Electronic Journal of …, 2022 - Elsevier
Background The temperature upshift has been widely used as an induction system to
produce recombinant proteins (RPs). However, thermoinduction could affect bacterial …
produce recombinant proteins (RPs). However, thermoinduction could affect bacterial …
Development of an Industrially Feasible Medium for Enhanced Production of Extremely Thermophilic Recombinant Endo-1,4-β-xylanase by Escherichia coli
Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding
gene isolated from an extremely thermophilic bacterium, Thermotoga neapolitana was used …
gene isolated from an extremely thermophilic bacterium, Thermotoga neapolitana was used …
Thermoinducible E. coli for Recombinant Protein Production in Inclusion Bodies
NA Valdez-Cruz, MA Trujillo-Roldán - Inclusion Bodies: Methods and …, 2023 - Springer
The temperature-inducible λ pL/pR-cI857 expression system has been widely used to
produce recombinant proteins (RPs), especially when it is necessary to avoid the addition of …
produce recombinant proteins (RPs), especially when it is necessary to avoid the addition of …
Maximization of saturated fatty acids through the production of P450BM3 monooxygenase in the engineered Escherichia coli
G Awad, A Garnier - Food and Bioproducts Processing, 2021 - Elsevier
The microbial fatty acid biosynthesis, which can be utilized as precursors for diverse
chemical production has recently attracted important attention. Saturated fatty acids (SFAs) …
chemical production has recently attracted important attention. Saturated fatty acids (SFAs) …
Effect of supplemented sugar in lysogeny broth medium on growth of Escherichia coli BL21(DE3) and recombinant protein production.
S Muenwongtham, P Jaturapiree, P Pimviriyakul - 2022 - cabidigitallibrary.org
Importance of the work: Escherichia coli BL21 (DE3) is among the powerful hosts for
recombinant protein expression. Varying approaches have been explored to improve the …
recombinant protein expression. Varying approaches have been explored to improve the …