DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

The polymerase chain reaction (PCR) is increasingly used as the standard method for
detection and characterization of microorganisms and genetic markers in a variety of sample …

In a recent study, immunoglobulin G in human plasma was identified as a major inhibitor of
diagnostic PCR (W. Abu Al-Soud, LJ Jönsson, and P. Rådström. J. Clin. Microbiol. 38 …

ABSTRACT A robust high-throughput multilocus sequence typing (MLST) scheme for
Clostridium difficile was developed and validated using a diverse collection of 50 reference …

Polymerase chain reaction (PCR) is recognized as a rapid, sensitive, and specific molecular
diagnostic tool for the analysis of nucleic acids. However, the sensitivity and kinetics of …

Polymerase chain reaction (PCR) is currently the method of choice for the identification of
human remains in forensic coursework. DNA samples from crime scenes often contain co …

Microbial pathogens are one of the major health risks associated with water and
wastewaters. Current methods for the detection of pathogenic viruses, bacteria, protozoa …

The PCR is an extremely powerful method for detecting microorganisms. However, its full
potential as a rapid detection method is limited by the inhibition of the thermostable DNA …

The purity of DNA extracted from faecal samples is a key issue in the sensitivity and
usefulness of biological analyses such as PCR for infectious pathogens and non-pathogens …

A completely revised and updated edition that teaches the essentials of forensic biology,
with increased coverage of molecular biological techniques and new information on wildlife …