Intrinsically disordered regions direct transcription factor in vivo binding specificity

S Brodsky, T Jana, K Mittelman, M Chapal, DK Kumar… - Molecular cell, 2020 - cell.com
Transcription factors (TFs) that bind common DNA motifs in vitro occupy distinct sets of
promoters in vivo, raising the question of how binding specificity is achieved. TFs are …

Multiscale models quantifying yeast physiology: towards a whole-cell model

H Lu, EJ Kerkhoven, J Nielsen - Trends in Biotechnology, 2022 - cell.com
The yeast Saccharomyces cerevisiae is widely used as a cell factory and as an important
eukaryal model organism for studying cellular physiology related to human health and …

Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator

AL Sanborn, BT Yeh, JT Feigerle, CV Hao… - Elife, 2021 - elifesciences.org
Gene activator proteins comprise distinct DNA-binding and transcriptional activation
domains (ADs). Because few ADs have been described, we tested domains tiling all yeast …

Global analysis of the yeast knockout phenome

G Turco, C Chang, RY Wang, G Kim, EH Stoops… - Science …, 2023 - science.org
Genome-wide phenotypic screens in the budding yeast Saccharomyces cerevisiae, enabled
by its knockout collection, have produced the largest, richest, and most systematic …

From classical mendelian randomization to causal networks for systematic integration of multi-omics

A Yazdani, A Yazdani, R Mendez-Giraldez… - Frontiers in …, 2022 - frontiersin.org
The number of studies with information at multiple biological levels of granularity, such as
genomics, proteomics, and metabolomics, is increasing each year, and a biomedical …

Inferring gene regulation from stochastic transcriptional variation across single cells at steady state

A Gupta, JD Martin-Rufino, TR Jones… - Proceedings of the …, 2022 - National Acad Sciences
Regulatory relationships between transcription factors (TFs) and their target genes lie at the
heart of cellular identity and function; however, uncovering these relationships is often labor …

Revisiting the model for coactivator recruitment: Med15 can select its target sites independent of promoter-bound transcription factors

V Mindel, S Brodsky, H Yung, W Manadre… - Nucleic Acids …, 2024 - academic.oup.com
Activation domains (ADs) within transcription factors (TFs) induce gene expression by
recruiting coactivators such as the Mediator complex. Coactivators lack DNA binding …

A genome‐scale yeast library with inducible expression of individual genes

Y Arita, G Kim, Z Li, H Friesen, G Turco… - Molecular Systems …, 2021 - embopress.org
The ability to switch a gene from off to on and monitor dynamic changes provides a powerful
approach for probing gene function and elucidating causal regulatory relationships. Here …

Bayesian inference of sample-specific coexpression networks

E Saha, V Fanfani, P Mandros, MB Guebila… - Genome …, 2024 - genome.cshlp.org
Gene regulatory networks (GRNs) are effective tools for inferring complex interactions
between molecules that regulate biological processes and hence can provide insights into …

[HTML][HTML] High-performance single-cell gene regulatory network inference at scale: the Inferelator 3.0

CS Gibbs, CA Jackson, GA Saldi, A Tjärnberg… - …, 2022 - ncbi.nlm.nih.gov
Results In this work, we present the Inferelator 3.0, which has been significantly updated to
integrate data from distinct cell types to learn context-specific regulatory networks and …