Deciphering molecular interactions by proximity labeling

W Qin, KF Cho, PE Cavanagh, AY Ting - Nature methods, 2021 - nature.com
Many biological processes are executed and regulated through the molecular interactions of
proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the …

Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide

S Zhang, JS Griffiths, G Marchand… - Molecular Plant …, 2022 - Wiley Online Library
Tomato brown rugose fruit virus (ToBRFV) is an emerging and rapidly spreading RNA virus
that infects tomato and pepper, with tomato as the primary host. The virus causes severe …

The MAPK-Alfin-like 7 module negatively regulates ROS scavenging genes to promote NLR-mediated immunity

D Zhang, Z Gao, H Zhang, Y Yang… - Proceedings of the …, 2023 - National Acad Sciences
Nucleotide-binding leucine-rich repeat (NLR) receptor-mediated immunity includes rapid
production of reactive oxygen species (ROS) and transcriptional reprogramming, which is …

Proximity labeling in mammalian cells with TurboID and split-TurboID

KF Cho, TC Branon, ND Udeshi, SA Myers, SA Carr… - Nature …, 2020 - nature.com
This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for mapping protein–protein interactions and subcellular proteomes in live …

Activation of TIR signalling boosts pattern-triggered immunity

H Tian, Z Wu, S Chen, K Ao, W Huang, H Yaghmaiean… - Nature, 2021 - nature.com
Plant immune responses are mainly activated by two types of receptor. Pattern recognition
receptors localized on the plasma membrane perceive extracellular microbial features, and …

Mapping the signaling network of BIN2 kinase using TurboID-mediated biotin labeling and phosphoproteomics

TW Kim, CH Park, CC Hsu, YW Kim, YW Ko… - The Plant …, 2023 - academic.oup.com
Elucidating enzyme–substrate relationships in posttranslational modification (PTM) networks
is crucial for understanding signal transduction pathways but is technically difficult because …

[HTML][HTML] EDS1 signalling: At the nexus of intracellular and surface receptor immunity

JA Dongus, JE Parker - Current opinion in plant biology, 2021 - Elsevier
The conserved lipase-like protein EDS1 transduces signals from pathogen-activated
intracellular nucleotide-binding leucine-rich repeat (NLR) receptors to transcriptional …

Plant immune signaling: Advancing on two frontiers

W Wang, B Feng, JM Zhou… - Journal of integrative plant …, 2020 - Wiley Online Library
Plants have evolved multiple defense strategies to cope with pathogens, among which plant
immune signaling that relies on cell‐surface localized and intracellular receptors takes …

Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID

A Mair, SL Xu, TC Branon, AY Ting, DC Bergmann - elife, 2019 - elifesciences.org
Defining specific protein interactions and spatially or temporally restricted local proteomes
improves our understanding of all cellular processes, but obtaining such data is challenging …

Origins and immunity networking functions of EDS1 family proteins

D Lapin, DD Bhandari, JE Parker - Annual review of …, 2020 - annualreviews.org
The EDS1 family of structurally unique lipase-like proteins EDS1, SAG101, and PAD4
evolved in seed plants, on top of existing phytohormone and nucleotide-binding–leucine …