Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis
thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and …

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short
palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR …

Recent advances with the type II clustered regularly interspaced short palindromic repeats
(CRISPR) system promise an improved approach to genome editing. However, the …

The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and
human disease with a rich array of in vivo genetic and molecular tools. However, the inability …

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for
targeted mutagenesis. Here we report a new class of sequence-specific nucleases created …

Figure 1 Target sequences, frequencies of mutations and mutations induced by TALEns in
embryonic zebrafish cells. For each pair of TALEns, the wild-type (WT) target sequence is …

We have previously reported a simple and customizable CRISPR (clustered regularly
interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can …

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we
describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only …

Abstract Customized TALENs and Cas9/gRNAs have been used for targeted mutagenesis in
zebrafish to induce indels into protein-coding genes. However, indels are usually not …