Virus-like particles in vaccine development

A Roldão, MCM Mellado, LR Castilho… - Expert review of …, 2010 - Taylor & Francis
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and
conformation of authentic native viruses but lack the viral genome, potentially yielding safer …

Large-scale production and purification of VLP-based vaccines

T Vicente, A Roldão, C Peixoto, MJT Carrondo… - Journal of invertebrate …, 2011 - Elsevier
Virus-like particles (VLPs) hold tremendous potential as vaccine candidates. These
innovative biopharmaceuticals present the remarkable advantages of closely mimicking the …

Insect cells as a production platform of complex virus-like particles

F Fernandes, AP Teixeira, N Carinhas… - Expert review of …, 2013 - Taylor & Francis
Virus-like particles (VLPs) are multiprotein structures that resemble the conformation of
native viruses but lack a viral genome, potentiating their application as safer and cheaper …

Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks

S Sokolenko, S George, A Wagner, A Tuladhar… - Biotechnology …, 2012 - Elsevier
The baculovirus expression vector system (BEVS) is a versatile and powerful platform for
protein expression in insect cells. With the ability to approach similar post-translational …

Improving baculovirus production at high cell density through manipulation of energy metabolism

N Carinhas, V Bernal, F Monteiro, MJT Carrondo… - Metabolic …, 2010 - Elsevier
The insect cells/baculovirus system is well recognized as a safe and suitable technology to
produce heterologous proteins, vaccines and vectors for gene therapy. Efficient and robust …

Error assessment in recombinant baculovirus titration: evaluation of different methods

A Roldão, R Oliveira, MJT Carrondo… - Journal of virological …, 2009 - Elsevier
The success of baculovirus/insect cells system in heterologous protein expression depends
on the robustness and efficiency of the production workflow. It is essential that process …

Challenges for the production of virus-like particles in insect cells: the case of rotavirus-like particles

LA Palomares, OT Ramírez - Biochemical Engineering Journal, 2009 - Elsevier
Virus-like particles (VLP) are formed when viral structural proteins are produced in an
heterologous expression system. Such proteins assemble into structures that are …

Process optimization for recombinant protein expression in insect cells

J Zitzmann, G Sprick, T Weidner… - New insights into cell …, 2017 - books.google.com
Insect cells can be used for the efficient production of heterologous proteins. The
baculovirus expression vector system (BEVS) in Spodoptera frugiperda cells and the stable …

[HTML][HTML] Viruses and virus-like particles in biotechnology: fundamentals and applications

A Roldão, AC Silva, MCM Mellado… - Comprehensive …, 2011 - ncbi.nlm.nih.gov
Although viruses are simple biological systems, they are capable of evolving highly efficient
techniques for infecting cells, expressing their genomes, and generating new copies of …

Quality control and analytical methods for baculovirus-based products

A Roldão, T Vicente, C Peixoto, MJT Carrondo… - Journal of invertebrate …, 2011 - Elsevier
Recombinant baculoviruses (rBac) are used for many different applications, ranging from bio-
insecticides to the production of heterologous proteins, high-throughput screening of gene …