In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …

Fluorescence fluctuation spectroscopy (FFS) techniques provide information at the single-
molecule level with excellent time resolution. Usually applied at a single spot in a sample …

Imaging mRNA with single-molecule sensitivity in live cells has become an indispensable
tool for quantitatively studying RNA biology. The MS2 system has been extensively used …

Specific binding proteins are crucial for the correct spatiotemporal expression of mRNA. To
understand this process, a method is required to characterize RNA-protein interactions in …

We demonstrate the calculation of particle brightness and concentration from fluorescence-
fluctuation photon-counting statistics using an electron-multiplied charge-coupled device …

In fluorescence microscopy, images often contain puncta in which the fluorescent molecules
are spatially clustered. This article describes a method that uses single-molecule intensity …

The red fluorescent protein mCherry is of considerable interest for fluorescence fluctuation
spectroscopy (FFS), because the wide separation in color between mCherry and green …

Mandel's Q-parameter, which is determined from the first two photon count moments,
provides an alternative to PCH analysis for determining the brightness of fluorophores. Here …

The experimental autocorrelation function of fluorescence correlation spectroscopy
calculated from finite-length data is a biased estimator of the theoretical correlation function …

Fluorescence correlation spectroscopy (FCS) measures rates of transport (diffusion
coefficients, convection velocities) and chemical reactions (rate constants) in equilibrium or …