Nanopore sequencing technology and its applications

P Zheng, C Zhou, Y Ding, B Liu, L Lu, F Zhu… - MedComm, 2023 - Wiley Online Library
Since the development of Sanger sequencing in 1977, sequencing technology has played a
pivotal role in molecular biology research by enabling the interpretation of biological genetic …

Enhancing Clinical Utility: Utilization of International Standards and Guidelines for Metagenomic Sequencing in Infectious Disease Diagnosis

CM Kan, HF Tsang, XM Pei, SSM Ng, AKY Yim… - International Journal of …, 2024 - mdpi.com
Metagenomic sequencing has emerged as a transformative tool in infectious disease
diagnosis, offering a comprehensive and unbiased approach to pathogen detection …

Comparative analysis of full-length 16s ribosomal RNA genome sequencing in human fecal samples using primer sets with different degrees of degeneracy

C Waechter, L Fehse, M Welzel, D Heider… - Frontiers in …, 2023 - frontiersin.org
Next-generation sequencing has revolutionized the field of microbiology research and
greatly expanded our knowledge of complex bacterial communities. Nanopore sequencing …

Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

E Buetas, M Jordán-López, A López-Roldán, G D'Auria… - BMC genomics, 2024 - Springer
Background Sequencing variable regions of the 16S rRNA gene (≃ 300 bp) with Illumina
technology is commonly used to study the composition of human microbiota. Unfortunately …

Long-read MinION™ sequencing of 16S and 16S-ITS-23S rRNA genes provides species-level resolution of Lactobacillaceae in mixed communities

SA Olivier, MK Bull, ML Strube, R Murphy… - Frontiers in …, 2023 - frontiersin.org
The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes
across numerous industries, and their unambiguous, species-level identification from mixed …

Breaking free from references: a consensus-based approach for community profiling with long amplicon nanopore data

W Stock, C Rousseau, G Dierickx… - Briefings in …, 2025 - academic.oup.com
Third-generation sequencing platforms, such as Oxford Nanopore Technology (ONT), have
made it possible to characterize communities through the sequencing of long amplicons …

A comprehensive review on probiotics and their use in aquaculture: Biological control, efficacy, and safety through the genomics and wet methods

M Calcagnile, SM Tredici, P Alifano - Heliyon, 2024 - cell.com
Probiotics, defined as viable microorganisms that enhance host health when consumed
through the diet, exert their effects through mechanisms such as strengthening the immune …

Insights into mycobacteriome composition in Mycobacterium bovis-infected African buffalo (Syncerus caffer) tissue samples

G Ghielmetti, TJ Kerr, N Bernitz, SK Mhlophe… - Scientific Reports, 2024 - nature.com
Animal tuberculosis significantly challenges global health, agriculture, and wildlife
conservation efforts. Mycobacterial cultures are resource-intensive, time-consuming, and …

Techniques, procedures, and applications in microbiome analysis

A González, A Fullaondo, A Odriozola - Advances in Genetics, 2024 - Elsevier
Microbiota is a complex community of microorganisms living in a defined environment. Until
the 20th century, knowledge of microbiota was partial, as the techniques available for their …

[PDF][PDF] Diversity Analysis of Fungi Distributed in Inhalable and Respirable Size Fractions of Aerosols: A Report from Kuwait

N Habibi, S Uddin, M Behbehani, M Kishk… - …, 2024 - researchgate.net
Fungi are an important part of the atmospheric ecosystem yet an underexplored group.
Airborne pathogenic fungi are the root cause of hypersensitive and allergenic states highly …