Lignin utilization: A review of lignin depolymerization from various aspects

C Chio, M Sain, W Qin - Renewable and sustainable energy reviews, 2019 - Elsevier
Lignin is the most abundant aromatic polymer in nature. Due to its high amount of phenolic
compounds storage, lignin is considered as an alternative source for various polymers and …

Lignin‐degrading enzymes

L Pollegioni, F Tonin, E Rosini - The FEBS journal, 2015 - Wiley Online Library
A main goal of green biotechnology is to reduce our dependence on fossil reserves and to
increase the use of renewable materials. For this, lignocellulose, which is composed of …

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

A Currin, N Swainston, PJ Day, DB Kell - Chemical Society Reviews, 2015 - pubs.rsc.org
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Hydrogen peroxide driven biocatalysis

BO Burek, S Bormann, F Hollmann, JZ Bloh… - Green …, 2019 - pubs.rsc.org
In general, hydrogen peroxide is a stable and relatively mild oxidant and it can be regarded
as the ultimate “green” reagent because water and oxygen are the only by-products …

Strategies for design of improved biocatalysts for industrial applications

A Madhavan, R Sindhu, P Binod, RK Sukumaran… - Bioresource …, 2017 - Elsevier
Biocatalysts are creating increased interest among researchers due to their unique
properties. Several enzymes are efficiently produced by microorganisms. However, the use …

Stable and functionally diverse versatile peroxidases designed directly from sequences

S Barber-Zucker, V Mindel, E Garcia-Ruiz… - Journal of the …, 2022 - ACS Publications
White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently
decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous …

Directed evolution of unspecific peroxygenase from Agrocybe aegerita

P Molina-Espeja, E Garcia-Ruiz… - Applied and …, 2014 - Am Soc Microbiol
Unspecific peroxygenase (UPO) represents a new type of heme-thiolate enzyme with self-
sufficient mono (per) oxygenase activity and many potential applications in organic …

Recent developments in the delignification and exploitation of grass lignocellulosic biomass

D Tocco, C Carucci, M Monduzzi, A Salis… - ACS Sustainable …, 2021 - ACS Publications
Biofuels could be defined as the fuels of the future, although much work is still required
before they will replace fossil fuels. In this review, lignocellulosic biomass based on straw …

Microbial lignin peroxidases: applications, production challenges and future perspectives

ODV Biko, M Viljoen-Bloom, WH van Zyl - Enzyme and Microbial …, 2020 - Elsevier
Lignin serves as the most abundant source of aromatic high-value products, but it has
remained underexploited due to its inert and recalcitrant nature. White-rot basidiomycetes …

Directed enzyme evolution: beyond the low-hanging fruit

M Goldsmith, DS Tawfik - Current opinion in structural biology, 2012 - Elsevier
The field of directed evolution has progressed to the point where it is feasible to engineer
enzymes for unnatural substrates and reactions with catalytic efficiencies and regio …