Formaldehyde crosslinking: a tool for the study of chromatin complexes
Formaldehyde has been used for decades to probe macromolecular structure and function
and to trap complexes, cells, and tissues for further analysis. Formaldehyde crosslinking is …
and to trap complexes, cells, and tissues for further analysis. Formaldehyde crosslinking is …
Using single‐cell genomics to understand developmental processes and cell fate decisions
High‐throughput‐omics techniques have revolutionised biology, allowing for thorough and
unbiased characterisation of the molecular states of biological systems. However, cellular …
unbiased characterisation of the molecular states of biological systems. However, cellular …
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA
CD Richardson, GJ Ray, MA DeWitt, GL Curie… - Nature …, 2016 - nature.com
Targeted genomic manipulation by Cas9 can efficiently generate knockout cells and
organisms via error-prone nonhomologous end joining (NHEJ), but the efficiency of precise …
organisms via error-prone nonhomologous end joining (NHEJ), but the efficiency of precise …
[HTML][HTML] Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair
I Psakhye, S Jentsch - Cell, 2012 - cell.com
Protein modification by SUMO affects a wide range of protein substrates. Surprisingly,
although SUMO pathway mutants display strong phenotypes, the function of individual …
although SUMO pathway mutants display strong phenotypes, the function of individual …
Highly expressed loci are vulnerable to misleading ChIP localization of multiple unrelated proteins
Chromatin immunoprecipitation (ChIP) is the gold-standard technique for localizing nuclear
proteins in the genome. We used ChIP, in combination with deep sequencing (Seq), to study …
proteins in the genome. We used ChIP, in combination with deep sequencing (Seq), to study …
[HTML][HTML] Acetylation is indispensable for p53 activation
The activation of the tumor suppressor p53 facilitates the cellular response to genotoxic
stress; however, the p53 response can only be executed if its interaction with its inhibitor …
stress; however, the p53 response can only be executed if its interaction with its inhibitor …
Uniform transitions of the general RNA polymerase II transcription complex
A Mayer, M Lidschreiber, M Siebert, K Leike… - Nature structural & …, 2010 - nature.com
We present genome-wide occupancy profiles for RNA polymerase (Pol) II, its
phosphorylated forms and transcription factors in proliferating yeast. Pol II exchanges …
phosphorylated forms and transcription factors in proliferating yeast. Pol II exchanges …
High-throughput chromatin immunoprecipitation for genome-wide mapping of in vivo protein-DNA interactions and epigenomic states
R Blecher-Gonen, Z Barnett-Itzhaki, D Jaitin… - Nature protocols, 2013 - nature.com
Dynamic protein binding to DNA elements regulates genome function and cell fate. Although
methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect …
methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect …
Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation
AA Joshi, K Struhl - Molecular cell, 2005 - cell.com
Eaf3, a component of the NuA4 histone acetylase and Rpd3 histone deacetylase
complexes, is important for the global pattern of histone acetylation in Saccharomyces …
complexes, is important for the global pattern of histone acetylation in Saccharomyces …
Epigenetic instability due to defective replication of structured DNA
P Sarkies, C Reams, LJ Simpson, JE Sale - Molecular cell, 2010 - cell.com
The accurate propagation of histone marks during chromosomal replication is proposed to
rely on the tight coupling of replication with the recycling of parental histones to the daughter …
rely on the tight coupling of replication with the recycling of parental histones to the daughter …