Formaldehyde has been used for decades to probe macromolecular structure and function
and to trap complexes, cells, and tissues for further analysis. Formaldehyde crosslinking is …

High‐throughput‐omics techniques have revolutionised biology, allowing for thorough and
unbiased characterisation of the molecular states of biological systems. However, cellular …

Targeted genomic manipulation by Cas9 can efficiently generate knockout cells and
organisms via error-prone nonhomologous end joining (NHEJ), but the efficiency of precise …

Protein modification by SUMO affects a wide range of protein substrates. Surprisingly,
although SUMO pathway mutants display strong phenotypes, the function of individual …

Chromatin immunoprecipitation (ChIP) is the gold-standard technique for localizing nuclear
proteins in the genome. We used ChIP, in combination with deep sequencing (Seq), to study …

The activation of the tumor suppressor p53 facilitates the cellular response to genotoxic
stress; however, the p53 response can only be executed if its interaction with its inhibitor …

We present genome-wide occupancy profiles for RNA polymerase (Pol) II, its
phosphorylated forms and transcription factors in proliferating yeast. Pol II exchanges …

Dynamic protein binding to DNA elements regulates genome function and cell fate. Although
methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect …

Eaf3, a component of the NuA4 histone acetylase and Rpd3 histone deacetylase
complexes, is important for the global pattern of histone acetylation in Saccharomyces …

The accurate propagation of histone marks during chromosomal replication is proposed to
rely on the tight coupling of replication with the recycling of parental histones to the daughter …