Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches

F Fuentes, E Munoz, MJ Contreras, ME Arias, R Felmer - Zygote, 2022 - cambridge.org
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique mainly used to
overcome severe infertility problems associated with the male factor, but in cattle its …

[HTML][HTML] Intracytoplasmic sperm injection in cattle

V Unnikrishnan, J Kastelic, J Thundathil - Genes, 2021 - mdpi.com
Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured
oocyte. Although this reproductive technology is successfully used in humans and many …

Sperm factors associated with the production of equine blastocysts by intracytoplasmic sperm injection (ICSI) using frozen/thawed semen

L Ramírez-Agámez, C Hernández-Avilés, DD Varner… - Theriogenology, 2023 - Elsevier
Abstract Intracytoplasmic Sperm Injection (ICSI) using frozen/thawed sperm is a common
procedure to obtain embryos from fertile or subfertile mares and stallions. Stallion …

[HTML][HTML] Effects of semen processing on sperm function: differences between swim-up and density gradient centrifugation

G Hernández-Silva, AS López-Torres… - The World Journal of …, 2021 - ncbi.nlm.nih.gov
Purpose Andrology research has evolved notoriously in the latest years, particularly since
male factor contribution to couple infertility has been undoubtedly demonstrated. However …

[HTML][HTML] Chromatin protamination and catsper expression in spermatozoa predict clinical outcomes after assisted reproduction programs

S Marchiani, L Tamburrino, F Benini, L Fanfani… - Scientific Reports, 2017 - nature.com
Identification of parameters predicting assisted reproductive technologies (ARTs) success is
a major goal of research in reproduction. Quality of gametes is essential to achieve good …

Significance of incorporating measures of sperm production and function into rat toxicology studies

SD Perreault, AM Cancel - Reproduction, 2001 - rep.bioscientifica.com
Studies have shown inhibitory effects of endogenous opioids on LH secretion in early post-
natal heifers. However, it is not clear whether these effects change during the rest of the …

Calcium chloride dihydrate supplementation at ICSI improves fertilization and pregnancy rates in patients with previous low fertilization: a retrospective paired …

S Popkiss, F Horta, B Vollenhoven, MP Green… - Journal of Assisted …, 2022 - Springer
Purpose To determine if 5mM calcium chloride dihydrate supplementation of the
Polyvinylpyrrolidone (PVP) media at the time of ICSI (ICSI-Ca) improves fertilization …

[HTML][HTML] Defective sperm head decondensation undermines the success of ICSI in the bovine

L Águila, R Felmer, ME Arias, F Navarrete… - …, 2017 - rep.bioscientifica.com
The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to
other species. It is unknown whether defective oocyte activation and/or sperm head …

In vitro production of (Farm) animal embryos

C Wrenzycki - Animal Biotechnology 1: Reproductive Biotechnologies, 2018 - Springer
Over the past decades, in vitro production (IVP) of bovine embryos has been significantly
improved, and in particular bovine IVP is now widely applied under field conditions. This in …

Haploid androgenetic development of bovine embryos reveals imbalanced WNT signaling and impaired cell fate differentiation

L Aguila, RP Nociti, RV Sampaio… - Biology of …, 2023 - academic.oup.com
Haploid embryos have contributed significantly to our understanding of the role of parental
genomes in development and can be applied to important biotechnology for human and …