The incorporation of a synthetic, catalytically competent metallocofactor into a protein
scaffold to generate an artificial metalloenzyme (ArM) has been explored since the late …

The field of artificial metalloenzymes (ArMs) is rapidly growing and ArMs are attracting
increasing attention, for example, in the fields of biosensing and drug therapy. Protein …

Conspectus In nature, heme cofactor-containing proteins participate not only in electron
transfer and O2 storage and transport but also in biosynthesis and degradation. The …

Artificial metalloenzymes combine the advantages of both natural enzymes and chemically
synthesized models, which have achieved significant progress in the last decade. This …

Incorporating artificial metal‐cofactors into protein scaffolds results in a new class of
catalysts, termed biohybrid catalysts or artificial metalloenzymes. Biohybrid catalysts can be …

Evolutionary engineering of our previously reported Cp* Rh (III)-linked artificial
metalloenzyme was performed based on a DNA recombination strategy to improve its …

Whole cell catalysis is, in many cases, a prerequisite for the cost-effective production of
chemicals by biotechnological means. Synthetic metal catalysts for bioorthogonal reactions …

Artifical metalloenzymes combine the reactivity of small molecule catalysts with the
selectivity of enzymes, and new methods are required to tune the catalytic properties of …

The hydrogen-evolving diiron complex,(μ-S) 2Fe2 (CO) 6 with a tethered maleimide moiety
was synthesized and covalently embedded within the cavity of a rigid β-barrel protein matrix …

Directed evolution has helped enzyme engineering to remarkable successes in the past. A
main challenge in directed evolution is to find the most suitable starting point, that is, an …