Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode
Abstract Background Plasmid DNA (pDNA) is a promising molecule for therapeutic
applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) …
applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) …
Plasmid DNA production for therapeutic applications
AR Lara, OT Ramírez - Recombinant Gene Expression, 2012 - Springer
Plasmid DNA (pDNA) is the base for promising DNA vaccines and gene therapies against
many infectious, acquired, and genetic diseases, including HIV-AIDS, Ebola, Malaria, and …
many infectious, acquired, and genetic diseases, including HIV-AIDS, Ebola, Malaria, and …
Sulfate limitation increases specific plasmid DNA yield and productivity in E. coli fed-batch processes
M Gotsmy, F Strobl, F Weiß, P Gruber, B Kraus… - Microbial Cell …, 2023 - Springer
Plasmid DNA (pDNA) is a key biotechnological product whose importance became apparent
in the last years due to its role as a raw material in the messenger ribonucleic acid (mRNA) …
in the last years due to its role as a raw material in the messenger ribonucleic acid (mRNA) …
De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production
GAL Gonçalves, DMF Prazeres, GA Monteiro… - Applied microbiology …, 2013 - Springer
The interest in plasmid DNA (pDNA) as a biopharmaceutical has been increasing over the
last several years, especially after the approval of the first DNA vaccines. New pDNA …
last several years, especially after the approval of the first DNA vaccines. New pDNA …
Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production
LG Fuentes, AR Lara, LM Martínez, OT Ramírez… - Microbial cell …, 2013 - Springer
Abstract Background The bacterium Escherichia coli can be grown employing various
carbohydrates as sole carbon and energy source. Among them, glucose affords the highest …
carbohydrates as sole carbon and energy source. Among them, glucose affords the highest …
Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains
Background Fed-batch mode is the standard culture technology for industrial bioprocesses.
Nevertheless, most of the early-stage cell and process development is carried out in batch …
Nevertheless, most of the early-stage cell and process development is carried out in batch …
Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing
GAL Goncalves, KLJ Prather, GA Monteiro… - Journal of …, 2014 - Elsevier
The market development of plasmid biopharmaceuticals for gene therapy and DNA
vaccination applications is critically dependent on the availability of cost-effective …
vaccination applications is critically dependent on the availability of cost-effective …
Aerobic expression of Vitreoscilla hemoglobin efficiently reduces overflow metabolism in Escherichia coli
TE Pablos, JC Sigala, S Le Borgne… - Biotechnology …, 2014 - Wiley Online Library
Overflow metabolism is a prevalent problem for aerobic cultivations of Escherichia coli.
Although several process and molecular approaches have been applied to prevent overflow …
Although several process and molecular approaches have been applied to prevent overflow …
Plasmid DNA Production in Proteome-Reduced Escherichia coli
The design of optimal cell factories requires engineering resource allocation for maximizing
product synthesis. A recently developed method to maximize the saving in cell resources …
product synthesis. A recently developed method to maximize the saving in cell resources …
Engineering E. coli for improved microaerobic pDNA production
KE Jaén, D Velazquez, F Delvigne, JC Sigala… - Bioprocess and …, 2019 - Springer
Escherichia coli strains W3110 and BL21 were engineered for the production of plasmid
DNA (pDNA) under aerobic and transitions to microaerobic conditions. The gene coding for …
DNA (pDNA) under aerobic and transitions to microaerobic conditions. The gene coding for …