DNA replication begins with the assembly of pre-replication complexes (pre-RCs) at
thousands of DNA replication origins during the G1 phase of the cell cycle. At the G1–S …

Transcriptional activation throughout the eukaryotic lineage has been tightly linked with
disruption of nucleosome organization at promoters, enhancers, silencers, insulators and …

Cellular DNA replication is initiated through the action of multiprotein complexes that
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …

Most current evidence indicates that DNA polymerases ε and δ, respectively, perform the
bulk of leading and lagging strand replication of the eukaryotic nuclear genome. Given that …

To unveil the still-elusive nature of metazoan replication origins, we identified them genome-
wide and at unprecedented high-resolution in mouse ES cells. This allowed initiation sites …

The size of a eukaryotic genome presents a unique challenge to the cell: package and
organize the DNA to fit within the confines of the nucleus while at the same time ensuring …

Mapping of nucleosomes, the basic DNA packaging unit in eukaryotes, is fundamental for
understanding genome regulation because nucleosomes modulate DNA access by their …

Complex regulation of gene expression in mammals has evolved from simpler eukaryotic
systems, yet the mechanistic features of this evolution remain elusive. Here, we compared …

Using a recently developed chemical approach, we have generated a genome-wide map of
nucleosomes in vivo in Schizosaccharomyces pombe (S. pombe) at base pair resolution …

Nucleosome positioning governs access to eukaryotic genomes. Many genes show a
stereotypic organisation at their 5′ end: a nucleosome free region just upstream of the …