The RaPID platform for the discovery of pseudo-natural macrocyclic peptides

Y Goto, H Suga - Accounts of Chemical Research, 2021 - ACS Publications
Conspectus Although macrocyclic peptides bearing exotic building blocks have proven their
utility as pharmaceuticals, the sources of macrocyclic peptide drugs have been largely …

Translation in prokaryotes

MV Rodnina - Cold Spring Harbor perspectives in …, 2018 - cshperspectives.cshlp.org
This review summarizes our current understanding of translation in prokaryotes, focusing on
the mechanistic and structural aspects of each phase of translation: initiation, elongation …

In vitro selection of macrocyclic peptide inhibitors containing cyclic γ2,4-amino acids targeting the SARS-CoV-2 main protease

T Miura, TR Malla, CD Owen, A Tumber, L Brewitz… - Nature Chemistry, 2023 - nature.com
Abstract γ-Amino acids can play important roles in the biological activities of natural
products; however, the ribosomal incorporation of γ-amino acids into peptides is …

Translation elongation and recoding in eukaryotes

TE Dever, JD Dinman, R Green - Cold Spring Harbor …, 2018 - cshperspectives.cshlp.org
In this review, we highlight the current understanding of translation elongation and recoding
in eukaryotes. In addition to providing an overview of the process, recent advances in our …

eIF5A functions globally in translation elongation and termination

AP Schuller, CCC Wu, TE Dever, AR Buskirk, R Green - Molecular cell, 2017 - cell.com
The eukaryotic translation factor eIF5A, originally identified as an initiation factor, was later
shown to promote translation elongation of iterated proline sequences. Using a combination …

Ribosomal synthesis and de novo discovery of bioactive foldamer peptides containing cyclic β-amino acids

T Katoh, T Sengoku, K Hirata, K Ogata, H Suga - Nature Chemistry, 2020 - nature.com
Peptides that contain β-amino acids display stable secondary structures, such as helices
and sheets, and are often referred to as foldamers. Cyclic β2, 3-amino acids (cβAAs), such …

Cell-free gene expression: methods and applications

AC Hunt, BJ Rasor, K Seki, HM Ekas, KF Warfel… - Chemical …, 2024 - ACS Publications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Structural basis for polyproline-mediated ribosome stalling and rescue by the translation elongation factor EF-P

P Huter, S Arenz, LV Bock, M Graf, JO Frister, A Heuer… - Molecular cell, 2017 - cell.com
Ribosomes synthesizing proteins containing consecutive proline residues become stalled
and require rescue via the action of uniquely modified translation elongation factors, EF-P in …

Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro

J Lee, KJ Schwarz, DS Kim, JS Moore… - Nature …, 2020 - nature.com
Ribosome-mediated polymerization of backbone-extended monomers into polypeptides is
challenging due to their poor compatibility with the translation apparatus, which evolved to …

In vitro genetic code reprogramming for the expansion of usable noncanonical amino acids

T Katoh, H Suga - Annual Review of Biochemistry, 2022 - annualreviews.org
Genetic code reprogramming has enabled us to ribosomally incorporate various
nonproteinogenic amino acids (npAAs) into peptides in vitro. The repertoire of usable npAAs …