Patterning of protein‐based materials

M Humenik, A Winkler, T Scheibel - Biopolymers, 2021 - Wiley Online Library
Micro‐and nanopatterning of proteins on surfaces allows to develop for example high‐
throughput biosensors in biomedical diagnostics and in general advances the …

Spidroin‐Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies

Q Zhang, M Li, W Hu, X Wang, J Hu - Stem Cells International, 2021 - Wiley Online Library
Spider silks are increasingly gaining interest for potential use as biomaterials in tissue
engineering and biomedical applications. Owing to their facile and versatile processability in …

Aptamer‐modified Nanohydrogel microarrays for bioselective cancer cell immobilization

Z Lamberger, H Bargel… - Advanced Functional …, 2022 - Wiley Online Library
Photolithography combined with surface nucleated protein self‐assembly of azido‐modified
spider silk proteins is used to create an arbitrarily shaped, inherently cell repellent …

DNA functionalized spider silk nanohydrogels for specific cell attachment and patterning

C Heinritz, Z Lamberger, K Kocourková, A Minarik… - ACS …, 2022 - ACS Publications
Nucleated protein self-assembly of an azido modified spider silk protein was employed in
the preparation of nanofibrillar networks with hydrogel-like properties immobilized on …

[HTML][HTML] Functionalized DNA-spider silk nanohydrogels for controlled protein binding and release

M Humenik, T Preiß, S Gödrich, G Papastavrou… - Materials Today Bio, 2020 - Elsevier
Hydrogels are excellent scaffolds to accommodate sensitive enzymes in a protective
environment. However, the lack of suitable immobilization techniques on substrates and the …

Dual patterning of self‐assembling spider silk protein nanofibrillar networks

Z Lamberger, K Kocourková, A Minařík… - Advanced Materials …, 2022 - Wiley Online Library
Self‐assembly of a recombinant spider silk protein into nanofibrillar networks in combination
with photolithography is used to produce diversely functionalized micropattern. Amino …

Conjugates of Recombinant Protein‐Based Polymers: Combining Precision with Chemical Diversity

D Hadar, DS Strugach, M Amiram - Advanced NanoBiomed …, 2022 - Wiley Online Library
Inspired by nature, scientists have harnessed the natural protein translation machinery to
produce intricate and precise protein‐based polymers (PBPs)—composed of natural amino …

Rapid Whole-Plate Cell and Tissue Micropatterning Using a Budget 3D Resin Printer

A Singh, YK Cho, DJ Cohen - ACS omega, 2024 - ACS Publications
The ability to precisely pattern cells and proteins is crucial in various scientific disciplines,
including cell biology, bioengineering, and materials chemistry. Current techniques, such as …

Effect of Terminal Modifications on the Adsorption and Assembly of hIAPP (20–29)

R Hajiraissi, M Hanke, A Gonzalez Orive, B Duderija… - ACS …, 2019 - ACS Publications
The assembly of peptides and proteins into nanoscale amyloid fibrils via formation of
intermolecular β-sheets not only plays an important role in the development of degenerative …

General Methods to Produce and Assemble Recombinant Spider Silk Proteins

N Kong - Fibrous Proteins: Design, Synthesis, and Assembly, 2021 - Springer
Orb-weaving spiders are known to spin up to seven types of silks/glues from different silk
glands. The inherent mechanical variety of these silks makes them attractive models for a …