[HTML][HTML] Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms

J Mao, H Zhang, Y Chen, L Wei, J Liu, J Nielsen… - Biotechnology …, 2024 - Elsevier
Metabolic burden is defined by the influence of genetic manipulation and environmental
perturbations on the distribution of cellular resources. The rewiring of microbial metabolism …

[HTML][HTML] New synthetic biology tools for metabolic control

X Lv, A Hueso-Gil, X Bi, Y Wu, Y Liu, L Liu… - Current Opinion in …, 2022 - Elsevier
In industrial bioprocesses, microbial metabolism dictates the product yields, and therefore,
our capacity to control it has an enormous potential to help us move towards a bio-based …

Light-driven CO2 sequestration in Escherichia coli to achieve theoretical yield of chemicals

G Hu, Z Li, D Ma, C Ye, L Zhang, C Gao, L Liu… - Nature Catalysis, 2021 - nature.com
CO2 sequestration engineering is an attractive strategy for achieving carbon-and energy-
efficient bioproduction. However, the efficiency of heterotrophic CO2 sequestration is limited …

Living intracellular inorganic-microorganism biohybrid system for efficient solar hydrogen generation

D Wu, W Zhang, B Fu, Z Zhang - Joule, 2022 - cell.com
The thirsty demand for renewable, sustainable, and efficient hydrogen production has
promoted the rapid development of advanced technologies, and whole-cell microorganism …

Reversible thermal regulation for bifunctional dynamic control of gene expression in Escherichia coli

X Wang, JN Han, X Zhang, YY Ma, Y Lin… - Nature …, 2021 - nature.com
Genetically programmed circuits allowing bifunctional dynamic regulation of enzyme
expression have far-reaching significances for various bio-manufactural purposes. However …

Engineering AraC to make it responsive to light instead of arabinose

E Romano, A Baumschlager, EB Akmeric… - Nature chemical …, 2021 - nature.com
The l-arabinose-responsive AraC and its cognate PBAD promoter underlie one of the most
often used chemically inducible prokaryotic gene expression systems in microbiology and …

Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae

H Liu, P Zhou, M Qi, L Guo, C Gao, G Hu… - Nature …, 2022 - nature.com
Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels.
However, product toxicity has hindered improvements in biofuel production. Here, we …

Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives

R Ohlendorf, A Möglich - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Numerous photoreceptors and genetic circuits emerged over the past two decades and now
enable the light-dependent ie, optogenetic, regulation of gene expression in bacteria …

The promise of optogenetics for bioproduction: dynamic control strategies and scale-up instruments

S Pouzet, A Banderas, M Le Bec, T Lautier, G Truan… - Bioengineering, 2020 - mdpi.com
Progress in metabolic engineering and synthetic and systems biology has made
bioproduction an increasingly attractive and competitive strategy for synthesizing …

Optogenetics in bacteria–applications and opportunities

F Lindner, A Diepold - FEMS Microbiology Reviews, 2022 - academic.oup.com
Optogenetics holds the promise of controlling biological processes with superb temporal
and spatial resolution at minimal perturbation. Although many of the light-reactive proteins …