The continuous flow synthesis of active pharmaceutical ingredients, value-added chemicals,
and materials has grown tremendously over the past ten years. This revolution in chemical …

The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

His‐tag affinity purification is one of the most commonly used methods to purify recombinant
proteins expressed in E. coli. One drawback of using the His‐tag is the co‐purification of …

Escherichia coli strains are widely used in academic research and biotechnology. New
technologies for quantifying strain-specific differences and their underlying contributing …

Virtually all recombinant proteins are now prepared using fusion domains also known as
“tags”. The use of tags helps to solve some serious problems: to simplify procedures of …

Membraneless organelles, like membrane-bound organelles, are essential to cell
homeostasis and provide discrete cellular subcompartments. Unlike classical organelles …

Electroporation is commonly used to deliver molecules such as drugs, proteins, and/or DNA
into cells, but the mechanism remains poorly understood. In this work a rapid microfluidic …

Coiled-coil (CC) dimers are versatile, customizable building modules for the design of
diverse protein architectures unknown in nature. Incorporation of dynamic self-assembly …

Hydroxytyrosol is a valuable plant-derived phenolic compound with excellent
pharmacological activities for application in the food and health care industries. Microbial …

Uncontrolled bone morphogenetic protein‐2 (BMP‐2) release can lead to off‐target bone
growth and other adverse events. To tackle this challenge, yeast surface display is used to …