A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
Enabling technology and core theory of synthetic biology
Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
RW Martin, BJ Des Soye, YC Kwon, J Kay… - Nature …, 2018 - nature.com
Cell-free protein synthesis has emerged as a powerful approach for expanding the range of
genetically encoded chemistry into proteins. Unfortunately, efforts to site-specifically …
genetically encoded chemistry into proteins. Unfortunately, efforts to site-specifically …
On-demand biomanufacturing of protective conjugate vaccines
Conjugate vaccines are among the most effective methods for preventing bacterial
infections. However, existing manufacturing approaches limit access to conjugate vaccines …
infections. However, existing manufacturing approaches limit access to conjugate vaccines …
Advances in cell‐free biosensors: principle, mechanism, and applications
L Zhang, W Guo, Y Lu - Biotechnology Journal, 2020 - Wiley Online Library
Synthetic biology has promoted the development of biosensors as tools for detecting trace
substances. In the past, biosensors based on synthetic biology have been designed on …
substances. In the past, biosensors based on synthetic biology have been designed on …
Bottom-up construction of complex biomolecular systems with cell-free synthetic biology
N Laohakunakorn, L Grasemann… - … in bioengineering and …, 2020 - frontiersin.org
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
Synthetic biology goes cell-free
A Tinafar, K Jaenes, K Pardee - BMC biology, 2019 - Springer
Cell-free systems (CFS) have recently evolved into key platforms for synthetic biology
applications. Many synthetic biology tools have traditionally relied on cell-based systems …
applications. Many synthetic biology tools have traditionally relied on cell-based systems …
A cell-free platform for the prenylation of natural products and application to cannabinoid production
MA Valliere, TP Korman, NB Woodall… - Nature …, 2019 - nature.com
Prenylation of natural compounds adds structural diversity, alters biological activity, and
enhances therapeutic potential. Because prenylated compounds often have a low natural …
enhances therapeutic potential. Because prenylated compounds often have a low natural …
[HTML][HTML] 合成生物学重要研究方向进展
丁明珠, 李炳志, 王颖, 谢泽雄, 刘夺, 元英进 - 合成生物学, 2020 - synbioj.cip.com.cn
摘要合成生物学作为一个新兴的交叉学科领域, 随着DNA 合成技术的进步和合成生物学理念的
深入, 多个研究方向取得了长足发展. 本文主要对基因回路, 基因组设计合成 …
深入, 多个研究方向取得了长足发展. 本文主要对基因回路, 基因组设计合成 …
Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems
The intricate and complex features of enzymatic reaction networks (ERNs) play a key role in
the emergence and sustenance of life. Constructing such networks in vitro enables stepwise …
the emergence and sustenance of life. Constructing such networks in vitro enables stepwise …