Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have …
associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have …
Inhibitors of bacterial immune systems: discovery, mechanisms and applications
D Mayo-Muñoz, R Pinilla-Redondo… - Nature Reviews …, 2024 - nature.com
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
[HTML][HTML] Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
[HTML][HTML] A compact, high-accuracy Cas9 with a dinucleotide PAM for in vivo genome editing
CRISPR-Cas9 genome editing has transformed biotechnology and therapeutics. However,
in vivo applications of some Cas9s are hindered by large size (limiting delivery by adeno …
in vivo applications of some Cas9s are hindered by large size (limiting delivery by adeno …
Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems
Clustered regularly interspaced short palindromic repeats (CRISPR) together with their
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
[HTML][HTML] The resurgence of phage-based therapy in the era of increasing antibiotic resistance: from research progress to challenges and prospects
NJ Anyaegbunam, CC Anekpo… - Microbiological …, 2022 - Elsevier
Phage therapy was implemented almost a century ago but was subsequently abandoned
when antibiotics emerged. However, the rapid emergence of drug-resistant, which has …
when antibiotics emerged. However, the rapid emergence of drug-resistant, which has …
AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation
W Sun, Z Cheng, J Wang, J Yang, X Li… - Proceedings of the …, 2023 - National Acad Sciences
Anti-CRISPR (Acr) proteins are encoded by phages and other mobile genetic elements and
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is a broad-spectrum …
[HTML][HTML] Structures of Neisseria meningitidis Cas9 complexes in catalytically poised and anti-CRISPR-inhibited states
High-resolution Cas9 structures have yet to reveal catalytic conformations due to HNH
nuclease domain positioning away from the cleavage site. Nme1Cas9 and Nme2Cas9 are …
nuclease domain positioning away from the cleavage site. Nme1Cas9 and Nme2Cas9 are …
Tissue-restricted genome editing in vivo specified by microRNA-repressible anti-CRISPR proteins
CRISPR-Cas systems are bacterial adaptive immune pathways that have revolutionized
biotechnology and biomedical applications. Despite the potential for human therapeutic …
biotechnology and biomedical applications. Despite the potential for human therapeutic …