THE definition of cellular organelles has evolved over the last hundred years largely driven
by morphologic observations, but more recently has been supplemented and complemented …

N-myristoylation is an acylation process absolutely specific to the N-terminal amino acid
glycine in proteins. This maturation process concerns about a hundred proteins in lower and …

The hydrolysis of phosphatidylcholine by phospholipase D (PLD) results in the production of
phosphatidic acid and choline. An assay that uses an exogenous substrate was developed …

Using three different trans dominant mutants of bovine ARF1 affecting GDP exchange or
GTP hydrolysis we demonstrate the central role of ARF1 in controlling vesicular traffic from …

Wee1 is a protein kinase that negatively regulates p34cdc2 kinase activity. We have
identified a Saccharomyces cerevisiae wee1 homolog encoded by the SWE1 gene. SWE1 …

The biosynthetic sorting of hydrolases to the yeast vacuole involves transport along two
distinct routes referred to as the carboxypeptidase Y and alkaline phosphatase pathways. To …

A family of three structurally related proteins were cloned from human cDNA libraries by their
ability to interact preferentially with the activated form of human ADP-ribosylation factor 3 …

The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-
ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is …

ADP-ribosylation factor appears to regulate the budding of both COPI and clathrin-coated
transport vesicles from Golgi membranes. An arf1Δ synthetic lethal screen identified …

Small GTP-binding proteins such as ADP-ribosylation factor 1 (ARF1) and Sar1p regulate
the membrane association of coat proteins involved in intracellular membrane trafficking …