Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Small-molecule fluorogenic probes for mitochondrial nanoscale imaging

R Zhai, B Fang, Y Lai, B Peng, H Bai, X Liu… - Chemical Society …, 2023 - pubs.rsc.org
Mitochondria are inextricably linked to the development of diseases and cell metabolism
disorders. Super-resolution imaging (SRI) is crucial in enhancing our understanding of …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

Parameter-free image resolution estimation based on decorrelation analysis

A Descloux, KS Grußmayer, A Radenovic - Nature methods, 2019 - nature.com
Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

D Sage, TA Pham, H Babcock, T Lukes, T Pengo… - Nature …, 2019 - nature.com
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Technological advances in super-resolution microscopy to study cellular processes

C Bond, AN Santiago-Ruiz, Q Tang, M Lakadamyali - Molecular Cell, 2022 - cell.com
Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging

W Zhao, S Zhao, Z Han, X Ding, G Hu, L Qu… - Nature …, 2023 - nature.com
High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision
profiling in a wide range of biomedical applications. However, current SR methods require …

Extending resolution within a single imaging frame

E Torres-García, R Pinto-Cámara, A Linares… - Nature …, 2022 - nature.com
The resolution of fluorescence microscopy images is limited by the physical properties of
light. In the last decade, numerous super-resolution microscopy (SRM) approaches have …

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation

RF Laine, HS Heil, S Coelho, J Nixon-Abell… - Nature …, 2023 - nature.com
Live-cell super-resolution microscopy enables the imaging of biological structure dynamics
below the diffraction limit. Here we present enhanced super-resolution radial fluctuations …