RNA splicing by the spliceosome
ME Wilkinson, C Charenton… - Annual review of …, 2020 - annualreviews.org
The spliceosome removes introns from messenger RNA precursors (pre-mRNA). Decades
of biochemistry and genetics combined with recent structural studies of the spliceosome …
of biochemistry and genetics combined with recent structural studies of the spliceosome …
Splicing and transcription touch base: co-transcriptional spliceosome assembly and function
Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA
polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length …
polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length …
A unified mechanism for intron and exon definition and back-splicing
The molecular mechanisms of exon definition and back-splicing are fundamental
unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy …
unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy …
Excised linear introns regulate growth in yeast
Spliceosomal introns are ubiquitous non-coding RNAs that are typically destined for rapid
debranching and degradation. Here we describe 34 excised introns in Saccharomyces …
debranching and degradation. Here we describe 34 excised introns in Saccharomyces …
[HTML][HTML] Profiling lariat intermediates reveals genetic determinants of early and late co-transcriptional splicing
Long introns with short exons in vertebrate genes are thought to require spliceosome
assembly across exons (exon definition), rather than introns, thereby requiring transcription …
assembly across exons (exon definition), rather than introns, thereby requiring transcription …
SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast
TJ Carrocci, DM Zoerner, JC Paulson… - Nucleic acids …, 2017 - academic.oup.com
RNA and protein components of the spliceosome work together to identify the 5΄ splice site,
the 3΄ splice site, and the branchsite (BS) of nascent pre-mRNA. SF3b1 plays a key role in …
the 3΄ splice site, and the branchsite (BS) of nascent pre-mRNA. SF3b1 plays a key role in …
THUMPD2 catalyzes the N2-methylation of U6 snRNA of the spliceosome catalytic center and regulates pre-mRNA splicing and retinal degeneration
WQ Yang, JY Ge, X Zhang, WY Zhu, L Lin… - Nucleic Acids …, 2024 - academic.oup.com
The mechanisms by which the relatively conserved spliceosome manages the enormously
large number of splicing events that occur in humans (∼ 200 000 versus∼ 300 in yeast) are …
large number of splicing events that occur in humans (∼ 200 000 versus∼ 300 in yeast) are …
[HTML][HTML] RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
S Battaglia, M Lidschreiber, C Baejen, P Torkler… - Elife, 2017 - elifesciences.org
For transcription through chromatin, RNA polymerase (Pol) II associates with elongation
factors (EFs). Here we show that many EFs crosslink to RNA emerging from transcribing Pol …
factors (EFs). Here we show that many EFs crosslink to RNA emerging from transcribing Pol …
Extremely fast and incredibly close: cotranscriptional splicing in budding yeast
EWJ Wallace, JD Beggs - Rna, 2017 - rnajournal.cshlp.org
RNA splicing, an essential part of eukaryotic pre-messenger RNA processing, can be
simultaneous with transcription by RNA polymerase II. Here, we compare and review …
simultaneous with transcription by RNA polymerase II. Here, we compare and review …
[HTML][HTML] Spliceosome profiling visualizes operations of a dynamic RNP at nucleotide resolution
JE Burke, AD Longhurst, D Merkurjev, J Sales-Lee… - Cell, 2018 - cell.com
Tools to understand how the spliceosome functions in vivo have lagged behind advances in
the structural biology of the spliceosome. Here, methods are described to globally profile …
the structural biology of the spliceosome. Here, methods are described to globally profile …