Mechanisms underlying spindle assembly and robustness

VA Valdez, L Neahring, S Petry… - Nature Reviews Molecular …, 2023 - nature.com
The microtubule-based spindle orchestrates chromosome segregation during cell division.
Following more than a century of study, many components and pathways contributing to …

Thirty years of search and capture: The complex simplicity of mitotic spindle assembly

R Heald, A Khodjakov - Journal of Cell Biology, 2015 - rupress.org
Cell division is enacted by a microtubule-based, self-assembling macromolecular machine
known as the mitotic spindle. In 1986, Kirschner and Mitchison proposed that by undergoing …

Regulation of kinetochore–microtubule attachments through homeostatic control during mitosis

KM Godek, L Kabeche, DA Compton - Nature Reviews Molecular Cell …, 2015 - nature.com
Faithful chromosome segregation during mitosis is essential for genome integrity and is
mediated by the bi-oriented attachment of replicated chromosomes to spindle microtubules …

Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis

C Sacristan, MUD Ahmad, J Keller, J Fermie… - Nature cell …, 2018 - nature.com
Faithful chromosome segregation depends on the ability of sister kinetochores to attach to
spindle microtubules. The outer layer of kinetochores transiently expands in early mitosis to …

Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces

M Barisic, P Aguiar, S Geley, H Maiato - Nature cell biology, 2014 - nature.com
Accurate chromosome segregation during cell division in metazoans relies on proper
chromosome congression at the equator. Chromosome congression is achieved after bi …

Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins

Y Steblyanko, G Rajendraprasad, M Osswald… - The EMBO …, 2020 - embopress.org
Mitotic spindle microtubules (MT s) undergo continuous poleward flux, whose driving force
and function in humans remain unclear. Here, we combined loss‐of‐function screenings …

Structure of the Ndc80 complex and its interactions at the yeast kinetochore–microtubule interface

JA Zahm, S Jenni, SC Harrison - Open biology, 2023 - royalsocietypublishing.org
The conserved Ndc80 kinetochore complex, Ndc80c, is the principal link between mitotic
spindle microtubules and centromere-associated proteins. We used AlphaFold 2 (AF2) to …

[HTML][HTML] Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells

RL Shrestha, GS Ahn, MI Staples, KM Sathyan… - Oncotarget, 2017 - ncbi.nlm.nih.gov
Chromosomal instability (CIN) is a hallmark of many cancers and a major contributor to
tumorigenesis. Centromere and kinetochore associated proteins such as the evolutionarily …

Phosphatases in mitosis: roles and regulation

M Moura, C Conde - Biomolecules, 2019 - mdpi.com
Mitosis requires extensive rearrangement of cellular architecture and of subcellular
structures so that replicated chromosomes can bind correctly to spindle microtubules and …

[HTML][HTML] Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression

J Wu, A Larreategui-Aparicio, MLA Lambers, DL Bodor… - Current Biology, 2023 - cell.com
Error-free chromosome segregation in mitosis and meiosis relies on the assembly of a
microtubule-based spindle that interacts with kinetochores to guide chromosomes to the cell …