FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

E Lerner, A Barth, J Hendrix, B Ambrose, V Birkedal… - Elife, 2021 - elifesciences.org
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Decoding and recoding of mRNA sequences by the ribosome

MV Rodnina - Annual Review of Biophysics, 2023 - annualreviews.org
Faithful translation of messenger RNA (mRNA) into protein is essential to maintain protein
homeostasis in the cell. Spontaneous translation errors are very rare due to stringent …

[HTML][HTML] Visualization of translation and protein biogenesis at the ER membrane

M Gemmer, ML Chaillet, J van Loenhout… - Nature, 2023 - nature.com
The dynamic ribosome–translocon complex, which resides at the endoplasmic reticulum
(ER) membrane, produces a major fraction of the human proteome,. It governs the synthesis …

Structure of the bacterial ribosome at 2 Å resolution

ZL Watson, FR Ward, R Méheust, O Ad, A Schepartz… - elife, 2020 - elifesciences.org
Using cryo-electron microscopy (cryo-EM), we determined the structure of the Escherichia
coli 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous …

[HTML][HTML] Visualizing translation dynamics at atomic detail inside a bacterial cell

L Xue, S Lenz, M Zimmermann-Kogadeeva, D Tegunov… - Nature, 2022 - nature.com
Translation is the fundamental process of protein synthesis and is catalysed by the ribosome
in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram …

Multicolor single-molecule FRET for DNA and RNA processes

XA Feng, MF Poyton, T Ha - Current opinion in structural biology, 2021 - Elsevier
Single-molecule fluorescence resonance energy transfer (smFRET) is a useful tool for
observing the dynamics of protein–nucleic acid interactions. Although most smFRET …

The Valsa Mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple

R Che, C Liu, Q Wang, W Tu, P Wang, C Li, X Gong… - Autophagy, 2023 - Taylor & Francis
Macroautophagy/autophagy is a conserved degradation pathway in eukaryotes that is
required for recycling unwanted intracellular components, maintaining homeostasis, and …

Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes

MF Juette, JD Carelli, EJ Rundlet, A Brown, S Shao… - Elife, 2022 - elifesciences.org
Rapid and accurate mRNA translation requires efficient codon-dependent delivery of the
correct aminoacyl-tRNA (aa-tRNA) to the ribosomal A site. In mammals, this fidelity …

cAMP binding to closed pacemaker ion channels is non-cooperative

DS White, S Chowdhury, V Idikuda, R Zhang… - Nature, 2021 - nature.com
Electrical activity in the brain and heart depends on rhythmic generation of action potentials
by pacemaker ion channels (HCN) whose activity is regulated by cAMP binding. Previous …

Getting to the bottom of lncRNA mechanism: structure–function relationships

K Sanbonmatsu - Mammalian Genome, 2022 - Springer
While long non-coding RNAs are known to play key roles in disease and development,
relatively few structural studies have been performed for this important class of RNAs. Here …