FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Decoding and recoding of mRNA sequences by the ribosome
MV Rodnina - Annual Review of Biophysics, 2023 - annualreviews.org
Faithful translation of messenger RNA (mRNA) into protein is essential to maintain protein
homeostasis in the cell. Spontaneous translation errors are very rare due to stringent …
homeostasis in the cell. Spontaneous translation errors are very rare due to stringent …
[HTML][HTML] Visualization of translation and protein biogenesis at the ER membrane
M Gemmer, ML Chaillet, J van Loenhout… - Nature, 2023 - nature.com
The dynamic ribosome–translocon complex, which resides at the endoplasmic reticulum
(ER) membrane, produces a major fraction of the human proteome,. It governs the synthesis …
(ER) membrane, produces a major fraction of the human proteome,. It governs the synthesis …
Structure of the bacterial ribosome at 2 Å resolution
ZL Watson, FR Ward, R Méheust, O Ad, A Schepartz… - elife, 2020 - elifesciences.org
Using cryo-electron microscopy (cryo-EM), we determined the structure of the Escherichia
coli 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous …
coli 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous …
[HTML][HTML] Visualizing translation dynamics at atomic detail inside a bacterial cell
Translation is the fundamental process of protein synthesis and is catalysed by the ribosome
in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram …
in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram …
Multicolor single-molecule FRET for DNA and RNA processes
Single-molecule fluorescence resonance energy transfer (smFRET) is a useful tool for
observing the dynamics of protein–nucleic acid interactions. Although most smFRET …
observing the dynamics of protein–nucleic acid interactions. Although most smFRET …
The Valsa Mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple
R Che, C Liu, Q Wang, W Tu, P Wang, C Li, X Gong… - Autophagy, 2023 - Taylor & Francis
Macroautophagy/autophagy is a conserved degradation pathway in eukaryotes that is
required for recycling unwanted intracellular components, maintaining homeostasis, and …
required for recycling unwanted intracellular components, maintaining homeostasis, and …
Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes
Rapid and accurate mRNA translation requires efficient codon-dependent delivery of the
correct aminoacyl-tRNA (aa-tRNA) to the ribosomal A site. In mammals, this fidelity …
correct aminoacyl-tRNA (aa-tRNA) to the ribosomal A site. In mammals, this fidelity …
cAMP binding to closed pacemaker ion channels is non-cooperative
Electrical activity in the brain and heart depends on rhythmic generation of action potentials
by pacemaker ion channels (HCN) whose activity is regulated by cAMP binding. Previous …
by pacemaker ion channels (HCN) whose activity is regulated by cAMP binding. Previous …
Getting to the bottom of lncRNA mechanism: structure–function relationships
K Sanbonmatsu - Mammalian Genome, 2022 - Springer
While long non-coding RNAs are known to play key roles in disease and development,
relatively few structural studies have been performed for this important class of RNAs. Here …
relatively few structural studies have been performed for this important class of RNAs. Here …