Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections

K Hsieh, JH Melendez, CA Gaydos, TH Wang - Lab on a Chip, 2022 - pubs.rsc.org
The incidence rates of sexually transmitted infections (STIs), including the four major curable
STIs–chlamydia, gonorrhea, trichomoniasis and, syphilis–continue to increase globally …

Point-of-care CRISPR-Cas-assisted SARS-CoV-2 detection in an automated and portable droplet magnetofluidic device

FE Chen, PW Lee, AY Trick, JS Park, L Chen… - Biosensors and …, 2021 - Elsevier
In the fight against COVID-19, there remains an unmet need for point-of-care (POC)
diagnostic testing tools that can rapidly and sensitively detect the causative SARS-CoV-2 …

Micro-and nanosystems for the detection of hemorrhagic fever viruses

M Bao, J Waitkus, L Liu, Y Chang, Z Xu, P Qin, J Chen… - Lab on a Chip, 2023 - pubs.rsc.org
Hemorrhagic fever viruses (HFVs) are virulent pathogens that can cause severe and often
fatal illnesses in humans. Timely and accurate detection of HFVs is critical for effective …

Chemical trends in sample preparation for nucleic acid amplification testing (NAAT): a Review

SM Lee, HK Balakrishnan, EH Doeven, D Yuan… - Biosensors, 2023 - mdpi.com
Nucleic acid amplification testing facilitates the detection of disease through specific
genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early …

Recent advances in digital biosensing technology

K Curtin, BJ Fike, B Binkley, T Godary, P Li - Biosensors, 2022 - mdpi.com
Digital biosensing assays demonstrate remarkable advantages over conventional
biosensing systems because of their ability to achieve single-molecule detection and …

Recent advances in magnetic nanoparticles-assisted microfluidic bioanalysis

Z Zhong, J He, G Li, L Xia - Chemosensors, 2023 - mdpi.com
Magnetic nanoparticles (MNPs) are attracting increasing attention in bioanalysis, due to their
large surface area and excellent steerable properties. Meanwhile, the booming …

Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration

A Traylor, PW Lee, K Hsieh, TH Wang - Analytica Chimica Acta, 2024 - Elsevier
Background Bacterial infections, especially polymicrobial infections, remain a threat to
global health and require advances in diagnostic technologies for timely and accurate …

Rapid automatic nucleic acid purification system based on gas–liquid immiscible phase

Y Fan, R Dai, X Guan, S Lu, C Yang… - Journal of Separation …, 2023 - Wiley Online Library
The continuous expansion of nucleic acid detection applications has resulted in constant
developments in rapid, low‐consumption, and highly automated nucleic acid extraction …

Facile Filtration-Based Workflow for Facilitating Sensitive Detection of Pathogenic Bacteria from Blood

DJM Park, L Chen, T Wu, PW Lee, K Hsieh, TH Wang - medRxiv, 2023 - medrxiv.org
Bloodstream infections (BSIs) are a global health concern, contributing to high mortality
rates and increased healthcare costs. Current diagnostic methods, such as blood culture …